Expression of H–2, laminin and SV40T and TASA on differentiation of transformed murine teratocarcinoma cells-猫眼课题宝

H-2、层粘连蛋白、SV40T和TASA在转化鼠畸胎癌细胞分化中的表达

DOI：

10.1038/288615a0

发表时间：

1980

期刊：

Nature

影响因子：

64.8

通讯作者：

中科院分区：

综合性期刊1区

文献类型：

作者：

Barbara B. Knowles;S. Pan;Davor Solter;Alban Linnenbach;Carlo M. Croce;Kay Huebner

小鼠胚胎癌细胞（ECCs）不表达主要组织相容性复合体（H-2）的抗原1，2，但表达与早期胚胎共有的细胞表面分子3。ECCs的特征还在于其对各种致癌病毒感染的不敏感性4 -7，以及其分化成各种成体细胞类型的能力8。体外ECCs的分化可以自发发生，也可以被诱导9 -12。在暴露于视黄酸时，ECC细胞系F9（参考文献13）分化为具有壁内胚层特征的细胞10，14。当ECCs暴露于猿猴病毒40（SV 40）时，SV 40肿瘤（T）抗原不表达4，尽管病毒基因组到达细胞核15，并产生SV 40 A基因的初级转录物16。然而，暴露于视黄酸后，分化的细胞，像大多数小鼠体细胞一样，对SV 40流产感染敏感，并合成大T和小t抗原17。为了监测分化时与SV 40 A基因表达相关的分子事件，我们构建了含有单个整合拷贝的SV 40基因组18的ECC系（F9 12-1）。这是通过将由连接至单纯疱疹1型胸苷激酶基因和SV 40基因组的pBR 322组成的重组质粒18引入胸苷激酶缺陷型F9细胞系19中来实现的。我们在此报道，在暴露于视黄酸的F9 12-1细胞中，SV 40 A基因产物T和肿瘤相关特异性抗原（TASA）的合成与该细胞系中正常分化标志H-2抗原和基底膜蛋白层粘连蛋白20的出现平行。

Murine embryonal carcinoma cells (ECCs) do not express antigens of the major histocompatibility complex (H–2)1,2, but do express cell-surface molecules shared with early embryos3. ECCs are also characterized by their insusceptibility to infection by various oncogenic viruses4-7, and their ability to differentiate into a variety of adult cell types8. Differentiation of ECCs in vitro can occur spontaneously or can be induced9–12. On exposure to retinoic acid the ECC line F9 (ref. 13) differentiates into cells which have the characteristics of parietal endoderm10,14. When ECCs are exposed to simian virus 40 (SV40), the SV40 tumour (T) antigen is not expressed4, although the virus genome reaches the nucleus15, and a primary transcript of the SV40 A gene is made16. However, following exposure to retinoic acid, the differentiated cells, like most mouse somatic cells, are susceptible to SV40 abortive infection and synthesize large T and small t antigens17. To monitor the molecular events associated with the expression of the SV40 A gene on differentiation, we have constructed an ECC line (F9 12–1) containing a single integrated copy of the SV40 genome18. This was accomplished by introducing a recombinant plasmid consisting of pBR322 linked to the herpes simplex type 1 thymidine kinase gene and SV40 genome18 into a thymidine kinase-deficient F9 cell line19. We report here that in F9 12–1 cells exposed to retinoic acid, synthesis of the SV40 A gene product(s), T and tumour-associated specific antigens (TASA), parallels the appearance of the normal hallmarks of differentiation in this cell line, H–2 antigens and the basement membrane protein laminin20.

DNA-transformed murine teratocarcinoma cells: regulation of expression of simian virus 40 tumor antigen in stem versus differentiated cells.