Fast benchtop fabrication of laminar flow chambers for advanced microscopy techniques.
Fast benchtop fabrication of laminar flow chambers for advanced microscopy techniques.
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DOI：
10.1371/journal.pone.0006479
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发表时间：
2009-08-03
期刊：
影响因子：
3.7
通讯作者：
Rock RS
中科院分区：
文献类型：
--
作者：
Courson DS;Rock RS
流体处理技术在实验室科学中的一个越来越重要的位置，无论是在简单的缓冲交换系统，灌注室还是先进的微流体设备中。这些应用中的许多应用仍然是大型机构实验室的天意，并具有大量的专业知识和专业设备。即使这些技术的扩展，局限性仍然存在，通常会阻止受控流体流与其他技术的耦合，例如微流体耦合以及高分辨率位置以及通过光学捕获显微镜进行的高分辨率位置和力测量。
在这里，我们提出了一种制造多输入层状流动设备的方法，这些方法在每个脸上是光学清晰的[玻璃]，化学惰性，可重复使用，便宜，并且可以在大约一小时内在台式上进行制造。此外，这些设备旨在通过简单的重力方法进行流量调节，因此不需要专门的设备来驱动流量。在这里，我们使用这些设备执行总内反射荧光显微镜测量以及位置敏感的光学陷阱实验。
普通科学界需要更容易获得流动室技术。此处介绍的方法是多功能且健壮的。这些设备使用标准幻灯片和盖玻片，使其与几乎所有类型的光显微镜兼容。这些设备满足了进行高级光学诱捕实验的组的需求，但也可以通过几乎所有具有与显微镜结合的溶液流量功能的实验室来调整。
Fluid handling technology is acquiring an ever more prominent place in laboratory science whether it is in simple buffer exchange systems, perfusion chambers, or advanced microfluidic devices. Many of these applications remain the providence of laboratories at large institutions with a great deal of expertise and specialized equipment. Even with the expansion of these techniques, limitations remain that frequently prevent the coupling of controlled fluid flow with other technologies, such as coupling microfluidics and high-resolution position and force measurements by optical trapping microscopy. Here we present a method for fabrication of multiple-input laminar flow devices that are optically clear [glass] on each face, chemically inert, reusable, inexpensive, and can be fabricated on the benchtop in approximately one hour. Further these devices are designed to allow flow regulation by a simple gravity method thus requiring no specialized equipment to drive flow. Here we use these devices to perform total internal reflection fluorescence microscopy measurements as well as position sensitive optical trapping experiments. Flow chamber technology needs to be more accessible to the general scientific community. The method presented here is versatile and robust. These devices use standard slides and coverslips making them compatible with nearly all types and models of light microscopes. These devices meet the needs of groups doing advanced optical trapping experiments, but could also be adapted by nearly any lab that has a function for solution flow coupled with microscopy.
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