Fluorescence polarization system for rapid COVID-19 diagnosis.
Fluorescence polarization system for rapid COVID-19 diagnosis.
复制标题
用于COVID-19快速诊断的荧光偏振系统。
DOI：
10.1016/j.bios.2021.113049
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发表时间：
2021-04-15
影响因子：
12.6
通讯作者：
Lee H
中科院分区：
文献类型：
--
作者：
Lee CY;Degani I;Cheong J;Lee JH;Choi HJ;Cheon J;Lee H
及时诊断、患者隔离和接触者追踪是遏制2019冠状病毒病（COVID-19）的关键措施。分子检测是目前COVID-19检测的金标准，但在中心实验室进行，延误了治疗和控制决策。在这里，我们描述了一种用于快速现场COVID-19诊断的便携式检测系统。该方法被称为CODA（CRISPR Optical Detection of Anisotropy），将等温核酸扩增、CRISPR/Cas12a的激活和信号生成结合在一起，消除了额外的手动步骤。重要的是，信号检测是基于荧光各向异性的比率测量，这使得CODA能够实现高信噪比。对于床旁操作，我们构建了一个紧凑的独立CODA设备，集成了光电器件、嵌入式加热器和用于数据处理的微控制器。该系统在上样20 min内完成了SARS-CoV-2 RNA的检测，检测限达到3 copy/μ L。当应用于临床样本（10名确诊的COVID-19患者; 10名对照）时，快速CODA测试准确地将COVID-19状态分类，与黄金标准临床诊断一致。
Prompt diagnosis, patient isolation, and contact tracing are key measures to contain the coronavirus disease 2019 (COVID-19). Molecular tests are the current gold standard for COVID-19 detection, but are carried out at central laboratories, delaying treatment and control decisions. Here we describe a portable assay system for rapid, onsite COVID-19 diagnosis. Termed CODA (CRISPR Optical Detection of Anisotropy), the method combined isothermal nucleic acid amplification, activation of CRISPR/Cas12a, and signal generation in a single assay, eliminating extra manual steps. Importantly, signal detection was based on the ratiometric measurement of fluorescent anisotropy, which allowed CODA to achieve a high signal-to-noise ratio. For point-of-care operation, we built a compact, standalone CODA device integrating optoelectronics, an embedded heater, and a microcontroller for data processing. The developed system completed SARS-CoV-2 RNA detection within 20 min of sample loading; the limit of detection reached 3 copy/μL. When applied to clinical samples (10 confirmed COVID-19 patients; 10 controls), the rapid CODA test accurately classified COVID-19 status, in concordance with gold-standard clinical diagnostics.
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影响因子：
16.6
作者：
Jeon Y;Choi YH;Jang Y;Yu J;Goo J;Lee G;Jeong YK;Lee SH;Kim IS;Kim JS;Jeong C;Lee S;Bae S
通讯作者：
Bae S
影响因子：
56.9
作者：
Chen, Janice S.;Ma, Enbo;Doudna, Jennifer A.
通讯作者：
Doudna, Jennifer A.
影响因子：
8.4
作者：
Rust, Philipp;Cereghetti, Damiano;Dual, Jurg
通讯作者：
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影响因子：
7.4
作者：
Zhang, Bing;Liu, Bingqian;Knopp, Dietmar
通讯作者：
Knopp, Dietmar
影响因子：
6.1
作者：
Rust, Philipp;Cereghetti, Damiano;Dual, Jurg
通讯作者：
Dual, Jurg