Rheological characterization of the bundling transition in F-actin solutions induced by methylcellulose.
Rheological characterization of the bundling transition in F-actin solutions induced by methylcellulose.
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DOI：
10.1371/journal.pone.0002736
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发表时间：
2008-07-16
期刊：
影响因子：
3.7
通讯作者：
Bausch AR
中科院分区：
文献类型：
--
作者：
Köhler S;Lieleg O;Bausch AR
在许多体外实验中，布朗运动阻碍了定量数据分析。因此，广泛使用添加剂来增加溶剂粘度。为此，甲基纤维素 (MC) 已被证明非常有效，因为很小的浓度就可以显着减慢扩散过程。除了这一优点之外，据报道，高 MC 浓度可以改变聚合物溶液（例如丝状肌动蛋白）的微观结构。然而，复合肌动蛋白/MC 凝胶的机械性能在多大程度上取决于 MC 浓度仍有待证明。特别是，即使微观结构似乎不受影响，也可能会发生重大变化。事实上，我们发现缠结的 F-肌动蛋白溶液的粘弹性响应敏感地取决于 MC 的添加量。当浓度高于 0.2% (w/v) MC 时，肌动蛋白丝会重组成束，从而极大地改变粘弹性响应。在较小的 MC 浓度下，MC 的影响更加微妙：肌动蛋白和 MC 这两种成分以累加的方式对复合材料的机械响应做出贡献。因此，当 MC 用于生化实验时，必须非常仔细地考虑甲基纤维素对肌动蛋白溶液的影响。
In many in vitro experiments Brownian motion hampers quantitative data analysis. Therefore, additives are widely used to increase the solvent viscosity. For this purpose, methylcellulose (MC) has been proven highly effective as already small concentrations can significantly slow down diffusive processes. Beside this advantage, it has already been reported that high MC concentrations can alter the microstructure of polymer solutions such as filamentous actin. However, it remains to be shown to what extent the mechanical properties of a composite actin/MC gel depend on the MC concentration. In particular, significant alterations might occur even if the microstructure seems unaffected. Indeed, we find that the viscoelastic response of entangled F-actin solutions depends sensitively on the amount of MC added. At concentrations higher than 0.2% (w/v) MC, actin filaments are reorganized into bundles which drastically changes the viscoelastic response. At small MC concentrations the impact of MC is more subtle: the two constituents, actin and MC, contribute in an additive way to the mechanical response of the composite material. As a consequence, the effect of methylcellulose on actin solutions has to be considered very carefully when MC is used in biochemical experiments.
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DOI：
10.1083/jcb.200603013
发表时间：
2006-09-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Vignjevic D;Kojima S;Aratyn Y;Danciu O;Svitkina T;Borisy GG
通讯作者：
Borisy GG
影响因子：
5.5
作者：
Kobayashi, K;Huang, CI;Lodge, TP
通讯作者：
Lodge, TP
影响因子：
3.4
作者：
Luan, Yuxia;Lieleg, Oliver;Bausch, Andreas R.
通讯作者：
Bausch, Andreas R.
影响因子：
9.2
作者：
Michelot, Alphee;Berro, Julien;Blanchoin, Laurent
通讯作者：
Blanchoin, Laurent
影响因子：
64.8
作者：
Loisel, TP;Boujemaa, R;Carlier, MF
通讯作者：
Carlier, MF