Role of fascin in filopodial protrusion.
Role of fascin in filopodial protrusion.
复制标题
DOI：
10.1083/jcb.200603013
复制
发表时间：
2006-09-11
期刊：
影响因子：
--
通讯作者：
Borisy GG
中科院分区：
文献类型：
--
作者：
Vignjevic D;Kojima S;Aratyn Y;Danciu O;Svitkina T;Borisy GG
本研究探讨了丝状足中肌动蛋白捆绑的机制。对小鼠黑色素瘤B16F1细胞中已知肌动蛋白交联蛋白的细胞定位分析显示，束状蛋白在所有丝状足的整个长度上都有特异性定位，而其他肌动蛋白交联蛋白则没有。RNA干扰筋膜蛋白使丝状足数量减少，剩余丝状足形态异常，呈波浪状，肌动蛋白组织松散捆绑。丝氨酸39的去磷酸化可能决定了细胞丝状伪足的频率。组成活性的束蛋白突变体S39A增加了丝足的数量和长度，而无活性的束蛋白突变体S39E减少了丝足的频率。gfp标记的野生型和S39A型束蛋白光漂白后的荧光恢复显示，去磷酸化的束蛋白与丝状足肌动蛋白丝的结合和分离周期非常快，周期为t1 /2 < 10 s。我们提出，束状蛋白是一种关键的特异性肌动蛋白交联剂，为丝状肌动蛋白束提供刚度，其动态行为允许丝状肌动蛋白丝的延伸和捆绑之间的有效协调。
In this study, the mechanisms of actin-bundling in filopodia were examined. Analysis of cellular localization of known actin cross-linking proteins in mouse melanoma B16F1 cells revealed that fascin was specifically localized along the entire length of all filopodia, whereas other actin cross-linkers were not. RNA interference of fascin reduced the number of filopodia, and remaining filopodia had abnormal morphology with wavy and loosely bundled actin organization. Dephosphorylation of serine 39 likely determined cellular filopodia frequency. The constitutively active fascin mutant S39A increased the number and length of filopodia, whereas the inactive fascin mutant S39E reduced filopodia frequency. Fluorescence recovery after photobleaching of GFP-tagged wild-type and S39A fascin showed that dephosphorylated fascin underwent rapid cycles of association to and dissociation from actin filaments in filopodia, with t 1/2 < 10 s. We propose that fascin is a key specific actin cross-linker, providing stiffness for filopodial bundles, and that its dynamic behavior allows for efficient coordination between elongation and bundling of filopodial actin filaments.
登录
查看更多内容
影响因子：
16.2
作者：
Lanier, LM;Gates, MA;Gertler, FB
通讯作者：
Gertler, FB
影响因子：
7.8
作者：
Bartles, J R;Zheng, L;Li, A;Wierda, A;Chen, B
通讯作者：
Chen, B
影响因子：
21.3
作者：
Berg, JS;Cheney, RE
通讯作者：
Cheney, RE
影响因子：
2.7
作者：
Kojima, SI;Vignjevic, D;Borisy, GG
通讯作者：
Borisy, GG
DOI：
10.1083/jcb.119.5.1219
发表时间：
1992-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lewis AK;Bridgman PC
通讯作者：
Bridgman PC