Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome.
Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome.
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DOI：
10.1016/s0140-6736(03)15259-2
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发表时间：
2004-01-10
期刊：
影响因子：
--
通讯作者：
Leung FC
中科院分区：
文献类型：
--
作者：
Guan Y;Peiris JS;Zheng B;Poon LL;Chan KH;Zeng FY;Chan CW;Chan MN;Chen JD;Chow KY;Hon CC;Hui KH;Li J;Li VY;Wang Y;Leung SW;Yuen KY;Leung FC
严重急性呼吸综合征（SARS）是由新型冠状病毒（SARS-CoV）引起的一种新型疾病，于2003年初在全球范围内蔓延，影响到30多个国家。我们利用分子流行病学来确定病毒在香港和其他地方的传播模式。SARS的病例定义是基于WHO推荐的病例定义。我们对2003年2月至4月香港（138例）和广东（3例）SARS患者的病毒刺突蛋白S1单位进行了基因测序。我们进行了系统发育比较与其他27个序列可从公共数据库（Genbank）。大部分的香港病毒（139/142），包括在一个公寓大厦爆发的大规模病毒，与一个单一的指示病例（HKU-33）的分离株紧密聚集在一起，该病例于2月底从广东来到香港。另外三个分离株在基因上与2月份在香港的HKU-33不同，但这些分离株都没有对随后的本地爆发造成重大影响。在广东和北京发现的病毒在基因上更加多样化。分子流行病学证据表明，香港爆发的大多数SARS冠状病毒，以及来自加拿大、越南和新加坡的病毒，在基因上是紧密相连的。2月份在香港发现的三种病毒在遗传学上与主要集群不同，这表明该病毒已经发生了几次引入，但只有一次与随后在香港爆发的疫情有关，而这又传播到全球。
Severe acute respiratory syndrome (SARS) is a newly emerged disease caused by a novel coronavirus (SARS-CoV), which spread globally in early 2003, affecting over 30 countries. We have used molecular epidemiology to define the patterns of spread of the virus in Hong Kong and beyond. The case definition of SARS was based on that recommended by WHO. We genetically sequenced the gene for the S1 unit of the viral spike protein of viruses from patients with SARS in Hong Kong (138) and Guangdong (three) in February to April, 2003. We undertook phylogenetic comparisons with 27 other sequences available from public databases (Genbank). Most of the Hong Kong viruses (139/142), including those from a large outbreak in an apartment block, clustered closely together with the isolate from a single index case (HKU-33) who came from Guangdong to Hong Kong in late February. Three other isolates were genetically distinct from HKU-33 in Hong Kong during February, but none of these contributed substantially to the subsequent local outbreak. Viruses identified in Guangdong and Beijing were genetically more diverse. The molecular epidemiological evidence suggests that most SARS-CoV from the outbreak in Hong Kong, as well as the viruses from Canada, Vietnam, and Singapore, are genetically closely linked. Three viruses found in Hong Kong in February were phylogenetically distinct from the major cluster, which suggests that several introductions of the virus had occurred, but that only one was associated with the subsequent outbreak in Hong Kong, which in turn spread globally.
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DOI：
10.1016/s0140-6736(03)14121-9
发表时间：
2003-08-16
期刊：
Lancet (London, England)
影响因子：
--
作者：
Ng SK
通讯作者：
Ng SK
DOI：
10.1016/s0140-6736(03)13077-2
发表时间：
2003-04-19
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Lai ST;Poon LL;Guan Y;Yam LY;Lim W;Nicholls J;Yee WK;Yan WW;Cheung MT;Cheng VC;Chan KH;Tsang DN;Yung RW;Ng TK;Yuen KY;SARS study group
通讯作者：
SARS study group
DOI：
10.1074/mcp.m300048-mcp200
发表时间：
2003-05
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Krokhin O;Li Y;Andonov A;Feldmann H;Flick R;Jones S;Stroeher U;Bastien N;Dasuri KV;Cheng K;Simonsen JN;Perreault H;Wilkins J;Ens W;Plummer F;Standing KG
通讯作者：
Standing KG
影响因子：
3.7
作者：
Gallagher TM;Buchmeier MJ
通讯作者：
Buchmeier MJ
影响因子：
5
作者：
Lee, CW;Jackwood, MW
通讯作者：
Jackwood, MW