Cloning of a Vero toxin (VT2) gene from a VT2‐converting phage isolated from Escherichia coli 0157: H7
Cloning of a Vero toxin (VT2) gene from a VT2‐converting phage isolated from Escherichia coli 0157: H7
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从大肠杆菌 0157: H7 分离的 VT2 转化噬菌体中克隆 Vero 毒素 (VT2) 基因
DOI：
10.1111/j.1574-6968.1987.tb02555.x
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发表时间：
1987
影响因子：
2.1
通讯作者：
Y. Takeda
中科院分区：
文献类型：
--
作者：
T. Yutsudo;H. Kurazono;C. Sasakawa;M. Yoshikawa;M. Iwaya;T. Takeda;Y. Takeda
从大肠杆菌0157：H7菌株J-2中分离到一种Vero毒素（VT 2或志贺样毒素II）转化噬菌体。非产毒大肠杆菌。大肠杆菌C600经毒素转化噬菌体溶原化后产生VT 2。将噬菌体DNA的EcoRI片段与EcoRI消化的pBR 322或pUC 118连接，转化E. coli MC 1061或MV 1184。表现出VT 2生产的转化体通常含有4.6kb的EcoRI片段。发现2.3kb的KpnI-SphI片段编码VT 2的产生，并且该片段与编码VT 1的2.1kb片段弱杂交。
A Vero toxin (VT2 or Shiga-like toxin II)-converting phage was isolated from Escherichia coli 0157: H7 strain J-2. Nontoxigenic E. coli C600 produced VT2 when lysogenized with the toxin-converting phage. EcoRI fragments of the phage DNA were ligated with EcoRI-digested pBR322 or pUC118 and were transformed into E. coli MC1061 or MV1184. Transformants exhibiting VT2 production commonly contained a 4.6 kb EcoRI fragment. It was found that a 2.3 kb KpnI-SphI fragment coded VT2 production and that this fragment hybridized weakly with the 2.1 kb fragment encoding VT1.
影响因子：
56.9
作者：
OBRIEN, AD;NEWLAND, JW;FORMAL, SB
通讯作者：
FORMAL, SB