Differential Regulation of Morphology and Estrogen Receptor-Alpha Expression in the Vagina of Ovariectomized Adult Virgin Rats by Estrogen Replacement: A Histological Study.
Differential Regulation of Morphology and Estrogen Receptor-Alpha Expression in the Vagina of Ovariectomized Adult Virgin Rats by Estrogen Replacement: A Histological Study.
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通过雌激素替代对切除卵巢的成年处女大鼠阴道形态和雌激素受体α表达的差异调节：组织学研究。
DOI：
10.1155/2016/1093512
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发表时间：
2016
影响因子：
2.8
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Li T;Ma Y;Zhang H;Yan P;Huo L;Hu Y;Chen X;Li T;Zhang M;Liu Z
背景确定雌激素在阴道组织形态学和雌激素受体α（ERα）在阴道中分布的确切作用，这一点仍然存在争议。方法.将60只大鼠随机分类：假手术（sham）、卵巢切除术（OVX）和4种雌二醇治疗（戊酸雌二醇0.4、0.8、1.6和3.2 mg/kg/天），持续2周。此后，从远侧和近侧半部分对阴道样本进行活检。采用免疫组化法定量ERα免疫反应细胞的百分比和ERα评分，以评估ERα表达和分布的变化。结果OVX引起明显的阴道萎缩和器官指数。雌激素替代疗法（ERT）逆转了阴道萎缩。阴道下半部ERα%低于下半部。OVX后4周ERα%显著升高，以上皮层ERα%升高最明显（P = 0.023）。治疗2周后，ERT组各组织ERα%均有不同程度的降低，但仅上皮ERα%与假手术组平行恢复（P = 0.001）。OVX组ERα组织学评分高于Sham组，远侧半区变化较近侧半区明显。ERT后ERα表达无明显变化（P > 0.05）。结论.雌激素替代治疗对绝经期妇女雌激素水平低下和年龄增长引起的肥胖和外阴阴道萎缩有效，但不能恢复ERα的分布和表达。
Background. To determine the exact role of estrogen in vaginal tissue morphology and estrogen receptor-alpha (ERα) distribution in the vagina, which remains controversial. Methods. Sixty rats were randomly categorized: sham-operated (sham), ovariectomy (OVX), and four estradiol treatments (estradiol valerate at 0.4, 0.8, 1.6, and 3.2 mg/kg/day) for 2 weeks. Thereafter, vaginal samples were biopsied from the distal- and proximal-half portions. The percentage of ERα-immunoreactive cells and the ERα score were quantified using immunohistochemistry to assess changes in ERα expression and distribution. Results. OVX induced significant vaginal atrophy and organic index. Estrogen-replacement therapy (ERT) reversed vaginal atrophy. The vaginal distal-half areas showed lower ERα% than the proximal-half areas. The ERα% increased sharply 4 weeks after OVX, especially in the epithelial layer (P = 0.023). ERT elicited different degrees of reductions in tissues after the 2-week treatment, but the ERα% in only the epithelium recovered in parallel with that in the sham group (P = 0.001). The OVX group showed higher ERα histological scores than the sham group, and the distal-half area changed more evidently than the proximal-half area. ERα expression was nearly unchanged after ERT (P > 0.05). Conclusions. ERT is effective for treating obesity and vulvovaginal atrophy caused by hypoestrogenism and advancing age in menopausal women but cannot recover the distribution and expression of ERα.
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影响因子：
8
作者：
Miyagawa, S;Katsu, Y;Iguchi, T
通讯作者：
Iguchi, T
影响因子：
6.7
作者：
Chen, GD;Oliver, RH;Yeh, O
通讯作者：
Yeh, O
影响因子：
4.8
作者：
Buckanan, DL;Kurita, T;Cooke, PS
通讯作者：
Cooke, PS
影响因子：
3.6
作者：
Cooke, PS;Buchanan, DL;Cunha, GR
通讯作者：
Cunha, GR
影响因子：
8.3
作者：
Rusa, R;Alkayed, NJ;Hurn, PD
通讯作者：
Hurn, PD