Inositol polyphosphate‐mediated iron transport in Pseudomonas aeruginosa
Inositol polyphosphate‐mediated iron transport in Pseudomonas aeruginosa
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铜绿假单胞菌中肌醇多磷酸介导的铁转运
DOI：
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发表时间：
1999
影响因子：
4
通讯作者：
A. W. Smith
中科院分区：
文献类型：
--
作者：
P. H. Hirst;A. M. Riley;S. J. Mills;I. Spiers;D. Poyner;S. Freeman;B. Potter;A. W. Smith
已有研究表明，肌醇六磷酸(myo-InsP6)介导铁向铜绿假单胞菌的转运，克服铁依赖的生长抑制。本研究研究了肌醇三磷酸和四磷酸肌醇区域异构体的铁转运特性。无论与myo-ins(1，2，3)P3或myo-InsP6络合，铜绿假单胞菌都以相似的速率积累铁(III)。其他化合物，特别是d/lmyo-ins(1，2，4，5)P4和另一种肌醇三磷酸区域异构体d-myo-ins(1，4，5)P3的铁积累显著增加。从myo-InsP6到缺少外膜孔蛋白oprF、oprD和oprP的突变体的铁转运谱与野生型相似，表明这些孔蛋白不参与外膜孔蛋白的转运过程。铁(III)还原成铁(II)的速率与任何一种化合物的络合。铜绿假单胞菌细胞裂解产物相似，表明还原机制不是速率决定步骤。
It has previously been shown that myo‐inositol hexakisphosphate (myo‐InsP6) mediates iron transport into Pseudomonas aeruginosa and overcomes iron‐dependent growth inhibition. In this study, the iron transport properties of myo‐inositol trisphosphate and tetrakisphosphate regio‐isomers were studied. Pseudomonas aeruginosa accumulated iron (III) at similar rates whether complexed with myo‐Ins(1,2,3)P3 or myo‐InsP6. Iron accumulation from other compounds, notably d/lmyo‐Ins(1,2,4,5)P4 and another inositol trisphosphate regio‐isomer, d‐myo‐Ins(1,4,5)P3, was dramatically increased. Iron transport profiles from myo‐InsP6 into mutants lacking the outer membrane porins oprF, oprD and oprP were similar to the wild‐type, indicating that these porins are not involved in the transport process. The rates of reduction of iron (III) to iron (II) complexed to any of the compounds by a Ps. aeruginosa cell lysate were similar, suggesting that a reductive mechanism is not the rate‐determining step.
影响因子：
7.1
作者：
HURRELL, RF;JUILLERAT, MA;COOK, JD
通讯作者：
COOK, JD
肌醇多磷酸受体和网格蛋白组装蛋白 AP-2 是在平面脂质双层中形成钾选择性离子通道的相关蛋白。
DOI：
10.1073/pnas.89.19.8976
发表时间：
1992
影响因子：
11.1
作者：
Timerman,AP;Mayrleitner,MM;Lukas,TJ;Chadwick,CC;Saito,A;Watterson,DM;Schindler,H;Fleischer,S
通讯作者：
Fleischer,S
肌醇六磷酸受体被鉴定为网格蛋白组装蛋白 AP-2。
DOI：
10.1016/s0006-291x(05)81473-1
发表时间：
1992
影响因子：
3.1
作者：
Voglmaier,SM;Keen,JH;Murphy,JE;Ferris,CD;Prestwich,GD;Snyder,SH;Theibert,AB
通讯作者：
Theibert,AB