Isolation and characterization of microsatellite markers for Cycas debaoensis Y. C. Zhong et C. J. Chen (Cycadaceae)
Isolation and characterization of microsatellite markers for Cycas debaoensis Y. C. Zhong et C. J. Chen (Cycadaceae)
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德保苏铁 (Cycas debaoensis) 微卫星标记的分离和鉴定（苏铁科）
DOI：
10.1111/j.1755-0998.2008.02114.x
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发表时间：
2008-07
影响因子：
7.7
通讯作者：
Yang, Yang
中科院分区：
文献类型：
--
作者：
Li, Lin-Feng;Ge, Xue-Jun;Gong, Xun;Li, Yong;Yang, Yang
德保苏铁是我国特有的极危苏铁种之一。在这项研究中，我们描述了来自C.利用AFLP快速分离重复序列（FIASCO）和来自表达序列标签数据库的两个微卫星标记（dbEST），对德保松叶蜂进行了遗传学分析。每个位点的多态性进行了评估，在60个成年个体的苏铁。每个微卫星位点的平均等位基因数为2.6个，从2到5不等。观察杂合度和期望杂合度分别为0.0833 ~ 0.7333和0.0805 ~ 0.7188。尽管Y177基因座很少见，但只有一个位点（Y177）由于过度纯合而偏离了Hardy-Weinberg平衡。对这8对引物在中国分布的其它4个同属种上进行了标记可转移性测试。
Cycas debaoensis is one of the critically endangered cycad species endemic to China. In this study, we described the development of six microsatellite markers from the genome of C. debaoensis using the protocol of fast isolation by AFLP of sequences containing repeats (FIASCO) and two microsatellite markers derived from the database of expressed sequence tags (dbEST). Polymorphism of each locus was assessed in 60 adult individuals of the cycad. The average allele number of the microsatellites was 2.6 per locus, ranging from two to five. The observed and expected heterozygosities varied from 0.0833 to 0.7333 and from 0.0805 to 0.7188, respectively. Despite its rarity, only one locus (Y177) deviated from Hardy–Weinberg equilibrium due to the excessive homozygosity. The marker transferability of the eight primer pairs was tested on other four congeneric species that also occur in China.
影响因子：
1.1
作者：
Jianguang Xie;Shu-guang Jian;L. Nian
通讯作者：
Jianguang Xie;Shu-guang Jian;L. Nian
影响因子：
3.1
作者：
Powell, W;Morgante, M;Rafalski, A
通讯作者：
Rafalski, A