Evaluation of liver fluke recombinant cathepsin B-1 protease as a serodiagnostic antigen for human opisthorchiasis.
Evaluation of liver fluke recombinant cathepsin B-1 protease as a serodiagnostic antigen for human opisthorchiasis.
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DOI：
10.1016/j.parint.2011.05.009
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发表时间：
2012-03
影响因子：
1.9
通讯作者：
Laha T
中科院分区：
文献类型：
--
作者：
Sripa J;Brindley PJ;Sripa B;Loukas A;Kaewkes S;Laha T
A cathepsin B-like cysteine protease belonging to family C1 is abundantly expressed in the transcriptome and proteome of the carcinogenic liver fluke of humans, Opisthorchis viverrini. This enzyme is present in excretory/secretory (ES) products released by parasites cultured in vitro. This study evaluated the performance of recombinant O. viverrini cathepsin B1 (rOv-CB-1) as an antigen for immunodiagnosis of opisthorchiasis. The full length Ov-CB-1 cDNA was cloned and recombinant protein was produced in catalytically active form in Pichia pastoris. The recombinant Ov-CB-1 (rOv-CB-1) was affinity purified via nickel-NTA chromatography and tested in enzyme-linked immunosorbent assays (ELISA) with human sera from an opisthorchiasis endemic area. Sera from egg-positive O. viverrini infections produced a strong IgG antibody response to rOv-CB-1 both in ELISA and immunoblot analysis. The sensitivity and specificity of the ELISA test was 67% and 81%, respectively. These findings support the feasibility of using recombinant Ov-CB-1 in ELISA for the serodiagnosis of human opisthorchiasis.
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影响因子：
15.8
作者：
Sripa, Banchob;Kaewkes, Sasithorn;Sithithaworn, Paiboon;Mairiang, Eimorn;Laha, Thewarach;Smout, Michael;Pairojkul, Chawalit;Bhudhisawasdi, Vajaraphongsa;Tesana, Smarn;Thinkamrop, Bandit;Bethony, Jeffrey M.;Loukas, Alex;Brindley, Paul J.
通讯作者：
Brindley, Paul J.
影响因子：
13.5
作者：
Sripa, Banchob;Mairiang, Eimorn;Thinkhamrop, Bandit;Laha, Thewarach;Kaewkes, Sasithorn;Sithithaworn, Paiboon;Tessana, Smarn;Loukas, Alex;Brindley, Paul J.;Bethony, Jeffrey M.
通讯作者：
Bethony, Jeffrey M.
影响因子：
8.4
作者：
Laha, Thewarach;Sripa, Jittiyawadee;Loukas, Alex
通讯作者：
Loukas, Alex
DOI：
10.4269/ajtmh.1988.38.356
发表时间：
1988-03-01
影响因子：
3.3
作者：
WONGRATANACHEEWIN, S;BUNNAG, D;SIRISINHA, S
通讯作者：
SIRISINHA, S
影响因子：
9.4
作者：
Bon, B.;Houze, S.;Hennequin, C.
通讯作者：
Hennequin, C.