Structural Elucidation of Cryptic Algaecides in Marine Algal-Bacterial Symbioses by NMR Spectroscopy and MicroED.
Structural Elucidation of Cryptic Algaecides in Marine Algal-Bacterial Symbioses by NMR Spectroscopy and MicroED.
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DOI：
10.1002/anie.202114022
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发表时间：
2022-01-21
影响因子：
16.6
通讯作者：
Seyedsayamdost, Mohammad R.
中科院分区：
文献类型：
--
作者：
Park, Jong-Duk;Li, Yuchen;Moon, Kyuho;Han, Esther J.;Lee, Seoung Rak;Seyedsayamdost, Mohammad R.
微生物次级代谢产物的发现通常在纯的单一培养物中进行。然而，在代谢物不断交换的自然环境中，生物合成前体可能由共生体或宿主提供。在目前的工作中，我们报告了八种新的和建筑上不寻常的次生代谢产物合成的细菌共生体Phaeacetylbens从前体，在本地的情况下，将提供他们的藻类宿主。其中三个是在低滴度和它们的结构从头确定使用新兴的微晶电子衍射方法。一些新的代谢产物表现出强大的杀藻活性，表明细菌共生体可以将藻类前体色氨酸和芥子酸转化为复杂的细胞毒素。我们的研究结果有重要的影响，寄生阶段的藻类-细菌共生相互作用。
Microbial secondary metabolite discovery is often conducted in pure monocultures. In a natural setting, however, where metabolites are constantly exchanged, bio-synthetic precursors are likely provided by symbionts or hosts. In the current work, we report eight novel and architecturally unusual secondary metabolites synthesized by the bacterial symbiont Phaeobacter inhibens from precursors that, in a native context, would be provided by their algal hosts. Three of these were produced at low titres and their structures were determined de novo using the emerging microcrystal electron diffraction method. Some of the new metabolites exhibited potent algaecidal activity suggesting that the bacterial symbiont can convert algal precursors, tryptophan and sinapic acid, into complex cytotoxins. Our results have important implications for the parasitic phase of algal-bacterial symbiotic interactions.
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影响因子：
15
作者：
Seyedsayamdost MR;Wang R;Kolter R;Clardy J
通讯作者：
Clardy J
影响因子：
3.3
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Nugroho, Alfarius Eko;Morita, Hiroshi
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作者：
Nett M;Ikeda H;Moore BS
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