Meningococcal carriage within households in the African meningitis belt: A longitudinal pilot study.
Meningococcal carriage within households in the African meningitis belt: A longitudinal pilot study.
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DOI：
10.1016/j.jinf.2017.11.006
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Sow SO
中科院分区：
文献类型：
--
作者：
Basta NE;Berthe A;Keita M;Onwuchekwa U;Tamboura B;Traore A;Hassan-King M;Manigart O;Nascimento M;Stuart JM;Trotter C;Blake J;Carr AD;Gray SJ;Newbold LS;Deng Y;Wolfson J;Halloran ME;Greenwood B;Borrow R;Sow SO
我们证明了在非洲脑膜炎带进行脑膜炎球菌携带的纵向、以家庭为基础的研究的可行性。在横断面筛查访问期间，携带流行率为5%（来自116个家庭的400名参与者中有20名携带者）。在17个月内，从20个家庭的202人中获得了1422份拭子。确定了73个携带者分离株; 33人（16.3%）至少携带一次。在采集的所有拭子中，84%的分离株是不可分组的，但鉴定出6株W和9株Y分离株。未发现A、C或X携带者。任何血清型的脑膜炎球菌携带时间为2.9个月（95%CI：1.6，5.4）。获得率为每月2.3%（95% CI：1.3，3.8）。脑膜炎奈瑟菌的携带者是传播的主要来源。在脑膜炎球菌病风险最高的非洲脑膜炎带，需要更好地了解脑膜炎球菌携带动态。我们从马里巴马科的116个家庭中随机选择了400名居民的年龄分层样本，并于2010年5月收集了咽拭子。一个月后，我们招募了其中20个家庭的所有202名居民（6名已知携带者），并在MenAfriVac疫苗引入前6个月每月收集拭子，10个月后返回，每月收集拭子，持续3个月。我们使用标准的细菌学方法鉴定N.脑膜炎病毒携带者，并拟合隐马尔可夫模型，以评估收购和清除的整体和性别和年龄。在横断面研究中，5.0%的个体（20/400）是携带者。在纵向研究期间，从分析的1422份拭子中确定了73起携带事件，16.3%的个体（33/202）至少一次被确定为携带者。大多数分离株是不可分组的;未发现血清群A携带者。我们的结果表明，任何N。脑膜炎平均发病2.9个月，马里城市地区的男性和儿童更容易怀孕和流产。我们的研究为在非洲脑膜炎带的七个国家实施的更大规模研究的设计提供了信息。
We demonstrated the feasibility of conducting a longitudinal, household-based study of meningococcal carriage in the African meningitis belt. During the cross-sectional screening visit, the carriage prevalence was 5% (20 carriers among 400 participants from 116 households). Over 17 months, 1422 swabs were obtained from 202 people in 20 households. 73 carrier isolates identified; 33 people (16.3%) carried at least once. Among all swabs collected, 84% of isolates were non-groupable, though 6 W and 9 Y isolates were identified. No A, C, or X carriers were found. The meningococcal carriage duration, any serogroup, was 2.9 months (95% CI: 1.6, 5.4). The acquisition rate was 2.3% per month (95% CI: 1.3, 3.8). Carriers of Neisseria meningitidis are a key source of transmission. In the African meningitis belt, where risk of meningococcal disease is highest, a greater understanding of meningococcal carriage dynamics is needed. We randomly selected an age-stratified sample of 400 residents from 116 households in Bamako, Mali, and collected pharyngeal swabs in May 2010. A month later, we enrolled all 202 residents of 20 of these households (6 with known carriers) and collected swabs monthly for 6 months prior to MenAfriVac vaccine introduction and returned 10 months later to collect swabs monthly for 3 months. We used standard bacteriological methods to identify N. meningitidis carriers and fit hidden Markov models to assess acquisition and clearance overall and by sex and age. During the cross-sectional study 5.0% of individuals (20/400) were carriers. During the longitudinal study, 73 carriage events were identified from 1422 swabs analyzed, and 16.3% of individuals (33/202) were identified as carriers at least once. The majority of isolates were non-groupable; no serogroup A carriers were identified. Our results suggest that the duration of carriage with any N. meningitidis averages 2.9 months and that males and children acquire and lose carriage more frequently in an urban setting in Mali. Our study informed the design of a larger study implemented in seven countries of the African meningitis belt.
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影响因子：
3.7
作者：
Basta, Nicole E.;Stuart, James M.;Greenwood, Brian
通讯作者：
Greenwood, Brian
影响因子：
9.4
作者：
Manigart, Olivier;Okeakpu, Jacinta;Greenwood, Brian M.
通讯作者：
Greenwood, Brian M.
影响因子：
--
作者：
Kristiansen, Paul A.;Diomande, Fabien;Caugant, Dominique A.
通讯作者：
Caugant, Dominique A.
DOI：
10.1093/infdis/jiv211
发表时间：
2015-10-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
MenAfriCar consortium
通讯作者：
MenAfriCar consortium
影响因子：
28.2
作者：
Jeppesen, Catherine A.;Snape, Matthew D.;Pollard, Andrew J.
通讯作者：
Pollard, Andrew J.