Identification of chromatophore membrane protein complexes formed under different nitrogen availability conditions in Rhodospirillum rubrum.

Identification of chromatophore membrane protein complexes formed under different nitrogen availability conditions in Rhodospirillum rubrum.
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DOI:
10.1021/pr100838x
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发表时间:
2011-06-03
影响因子:
4.4
通讯作者:
Noren, Agneta
Noren, Agneta
中科院分区:
生物学2区
文献类型:
--
作者:
Selao, Tiago Toscano;Branca, Rui;Chae, Pil Seok;Lehtio, Janne;Gellman, Samuel H.;Rasmussen, Soren G. F.;Nordlund, Stefan;Noren, Agneta

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光合固氮菌红色红杜鹃的色素细胞膜对固氮等许多中心过程具有重要作用。使用一种新的两亲物,我们已经确定了蛋白质复合物存在于不同的氮供应条件下,通过使用二维蓝色天然/ SDS-PAGE和NSI-LC-LTQ-轨道阱质谱。我们已经确定了几种膜蛋白复合物,包括ATP合成酶,反应中心,光捕获和NADH脱氢酶复合物的组件。此外,我们已经确定了差异表达的蛋白质,如琥珀酸脱氢酶复合物的亚基和其他TCA循环酶,通常在胞质溶胶中发现,从而暗示在一个可能的协会响应氮缺乏的膜。我们提出了一个氧化还原传感机制,可以影响膜亚蛋白质组响应氮的可用性。
The chromatophore membrane of the photosynthetic diazotroph Rhodospirillum rubrum is of vital importance for a number of central processes, including nitrogen fixation. Using a novel amphiphile, we have identified protein complexes present under different nitrogen availability conditions by the use of two-dimensional Blue Native / SDS-PAGE and NSI-LC-LTQ-Orbitrap mass spectrometry. We have identified several membrane protein complexes, including components of the ATP synthase, reaction center, light harvesting and NADH dehydrogenase complexes. Additionally, we have identified differentially expressed proteins, such as subunits of the succinate dehydrogenase complex and other TCA cycle enzymes that are usually found in the cytosol, thus hinting at a possible association to the membrane in response to nitrogen deficiency. We propose a redox sensing mechanism that can influence the membrane subproteome in response to nitrogen availability.
DOI: 10.1016/0005-2795(77)90274-4
发表时间: 1977-01-01
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