Identification of chromatophore membrane protein complexes formed under different nitrogen availability conditions in Rhodospirillum rubrum.
Identification of chromatophore membrane protein complexes formed under different nitrogen availability conditions in Rhodospirillum rubrum.
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DOI:
10.1021/pr100838x
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发表时间:
2011-06-03
影响因子:
4.4
通讯作者:
Noren, Agneta
中科院分区:
文献类型:
--
作者:
Selao, Tiago Toscano;Branca, Rui;Chae, Pil Seok;Lehtio, Janne;Gellman, Samuel H.;Rasmussen, Soren G. F.;Nordlund, Stefan;Noren, Agneta
The chromatophore membrane of the photosynthetic diazotroph Rhodospirillum rubrum is of vital importance for a number of central processes, including nitrogen fixation. Using a novel amphiphile, we have identified protein complexes present under different nitrogen availability conditions by the use of two-dimensional Blue Native / SDS-PAGE and NSI-LC-LTQ-Orbitrap mass spectrometry. We have identified several membrane protein complexes, including components of the ATP synthase, reaction center, light harvesting and NADH dehydrogenase complexes. Additionally, we have identified differentially expressed proteins, such as subunits of the succinate dehydrogenase complex and other TCA cycle enzymes that are usually found in the cytosol, thus hinting at a possible association to the membrane in response to nitrogen deficiency. We propose a redox sensing mechanism that can influence the membrane subproteome in response to nitrogen availability.
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DOI:
10.1016/0005-2795(77)90274-4
发表时间:
1977-01-01
期刊:
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子:
--
作者:
CHAI, TJ;FOULDS, J
通讯作者:
FOULDS, J
影响因子:
3.7
作者:
Fejes, AP;Yi, EC;Beatty, JT
通讯作者:
Beatty, JT
影响因子:
7.4
作者:
ANDERSON, L;FULLER, RC
通讯作者:
FULLER, RC
影响因子:
2.1
作者:
Brostedt, E;Lindblad, A;Nordlund, S
通讯作者:
Nordlund, S
影响因子:
2.9
作者:
Dunn, SD;Kellner, E;Lill, H
通讯作者:
Lill, H