Effects of bovine leukemia virus infection on milk neutrophil function and the milk lymphocyte profile.
Effects of bovine leukemia virus infection on milk neutrophil function and the milk lymphocyte profile.
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DOI：
10.1186/s13567-014-0125-4
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发表时间：
2015-01-17
影响因子：
4.4
通讯作者：
Blagitz MG
中科院分区：
文献类型：
--
作者：
Della Libera AM;de Souza FN;Batista CF;Santos BP;de Azevedo LF;Sanchez EM;Diniz SA;Silva MX;Haddad JP;Blagitz MG
牛白血病病毒(BLV)对免疫应答的影响已被广泛研究，但其对乳腺免疫的影响仅是推测。尽管BLV对B细胞有趋向性，但它可以影响获得性免疫和先天免疫，因为这些系统共享许多效应器机制。这一情景是研究BLV对乳腺免疫的影响的基础，乳腺免疫在很大程度上依赖于中性粒细胞功能。因此，本研究试图检测自然感染BLV的奶牛的乳中性粒细胞功能和淋巴细胞谱。感染BLV的奶牛和未感染BLV的奶牛乳中性粒细胞的活性和产生ROS或吞噬金黄色葡萄球菌的乳中性粒细胞百分比相似。此外，BLV感染奶牛乳汁中性粒细胞CD62L和CD11b的表达以及乳汁中性粒细胞(CH138+细胞)的百分率没有改变。相反，BLV感染奶牛，尤其是持续性淋巴细胞增多症(PL)奶牛的乳细胞中，代表细胞内ROS产生和金黄色葡萄球菌吞噬作用的中位荧光强度(MFI)、乳中性粒细胞CD44的表达和B细胞的凋亡率都较低。除CD5−/CD11b−B细胞比例较高外，其他各组间的淋巴细胞亚群差异不显著，尤其是PL奶牛的乳细胞。因此，本研究为BLV感染对乳腺免疫的影响提供了新的见解。
The effects of bovine leukemia virus (BLV) on the immune response have been extensively investigated; however, its effects on mammary gland immunity are only speculative. Although BLV has a tropism for B cells, it can affect both adaptive and innate immunities because these systems share many effector mechanisms. This scenario is the basis of this investigation of the effects of BLV on mammary gland immunity, which is largely dependent upon neutrophilic functions. Thus, the present study sought to examine neutrophilic functions and the lymphocyte profile in the milk of naturally BLV-infected cows. The viability of the milk neutrophils and the percentage of milk neutrophils that produced reactive oxygen species (ROS) or phagocytosed Staphylococcus aureus were similar between BLV-infected and BLV-uninfected dairy cows. Furthermore, the expression of CD62L and CD11b by the milk neutrophils and the percentage of milk neutrophils (CH138+ cells) that were obtained from the udder quarters of the BLV-infected cows were not altered. Conversely, the median fluorescence intensity (MFI) representing intracellular ROS production and the phagocytosis of S. aureus, the expression of CD44 by the milk neutrophils and the percentage of apoptotic B cells were lower in the milk cells from BLV-infected dairy cows, particularly those from animals with persistent lymphocytosis (PL). The lymphocyte subsets were not different among the groups, with the exception of the percentage of CD5−/CD11b− B cells, which was higher in the milk cells from BLV-infected cows, particularly those with PL. Thus, the present study provides novel insight into the implications of BLV infection for mammary gland immunity.
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DOI：
10.1097/01.qai.0000174252.73516.7a
发表时间：
2005-12-15
影响因子：
3.6
作者：
Marinho, J;Galvao-Castro, B;Barreto, ML
通讯作者：
Barreto, ML
影响因子：
3.1
作者：
Florins, Arnaud;Gillet, Nicolas;Willems, Luc
通讯作者：
Willems, Luc
影响因子：
--
作者：
Bojarojc-Nosowicz, B;Kaczmarczyk, E
通讯作者：
Kaczmarczyk, E
影响因子：
1
作者：
Juliarena, Marcela A.;Gutierrez, Silvina E.;Ceriani, Carolina
通讯作者：
Ceriani, Carolina
影响因子：
4.4
作者：
Ikebuchi R;Konnai S;Okagawa T;Yokoyama K;Nakajima C;Suzuki Y;Murata S;Ohashi K
通讯作者：
Ohashi K