Trans-species synthetic gene design allows resistance pyramiding and broad-spectrum engineering of virus resistance in plants.
Trans-species synthetic gene design allows resistance pyramiding and broad-spectrum engineering of virus resistance in plants.
复制标题
DOI：
10.1111/pbi.12896
复制
发表时间：
2018-03-05
影响因子：
13.8
通讯作者：
Gallois JL
中科院分区：
文献类型：
--
作者：
Bastet A;Lederer B;Giovinazzo N;Arnoux X;German-Retana S;Reinbold C;Brault V;Garcia D;Djennane S;Gersch S;Lemaire O;Robaglia C;Gallois JL
为了感染植物，病毒在很大程度上依赖于宿主的机器。基于宿主因子修饰的植物遗传抗性可以在现有的自然变异中找到，并且被广泛用于一些但不是所有作物。虽然生物技术可以弥补天然抗性等位基因的缺乏，但需要开发新的策略来增加抗性谱和耐久性，而不损害植物发育。在这里，我们评估了拟南芥翻译起始因子eIF4E1的靶向等位基因修饰如何导致对马铃薯Y病毒主要类群的广泛而有效的抗性。通过在天然存在的豌豆等位基因中引入与对马铃薯Y病毒的抗性相关的多个氨基酸变化来设计合成的拟南芥eIF4E1等位基因。这种新的等位基因编码一种功能蛋白，同时保持植物对通常劫持eIF4E1的马铃薯Y病毒属分离株的抗性。由于其生物学功能，这种合成等位基因允许在没有发育成本的情况下消除对马铃薯Y病毒的抗性，所述马铃薯Y病毒选择性地使用两种主要的翻译起始因子eIF4E1或其同种型eIFiso4E。此外，这种组合将耐药性谱扩展到迄今为止尚未发现有效耐药性的马铃薯Y病毒属分离株，包括耐药性破坏分离株和属于黄病毒科的不相关病毒。这项研究是基因工程与自然变异知识相结合的效率的概念验证，以产生跨物种病毒抗性，而不会对植物产生发育成本。这对使用最近的基因组编辑技术培育具有广谱和高持久性抗性的作物具有影响。
To infect plants, viruses rely heavily on their host's machinery. Plant genetic resistances based on host factor modifications can be found among existing natural variability and are widely used for some but not all crops. While biotechnology can supply for the lack of natural resistance alleles, new strategies need to be developed to increase resistance spectra and durability without impairing plant development. Here, we assess how the targeted allele modification of the Arabidopsis thaliana translation initiation factor eIF4E1 can lead to broad and efficient resistance to the major group of potyviruses. A synthetic Arabidopsis thaliana eIF4E1 allele was designed by introducing multiple amino acid changes associated with resistance to potyvirus in naturally occurring Pisum sativum alleles. This new allele encodes a functional protein while maintaining plant resistance to a potyvirus isolate that usually hijacks eIF4E1. Due to its biological functionality, this synthetic allele allows, at no developmental cost, the pyramiding of resistances to potyviruses that selectively use the two major translation initiation factors, eIF4E1 or its isoform eIFiso4E. Moreover, this combination extends the resistance spectrum to potyvirus isolates for which no efficient resistance has so far been found, including resistance‐breaking isolates and an unrelated virus belonging to the Luteoviridae family. This study is a proof‐of‐concept for the efficiency of gene engineering combined with knowledge of natural variation to generate trans‐species virus resistance at no developmental cost to the plant. This has implications for breeding of crops with broad‐spectrum and high durability resistance using recent genome editing techniques.
登录
查看更多内容
影响因子：
5
作者：
Beauchemin, C;Bougie, V;Laliberté, JF
通讯作者：
Laliberté, JF
影响因子：
7.2
作者：
Gauffier, Camille;Lebaron, Caroline;Gallois, Jean-Luc
通讯作者：
Gallois, Jean-Luc
影响因子：
7.2
作者：
Gao, ZH;Johansen, E;Maule, AJ
通讯作者：
Maule, AJ
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
4.9
作者：
Chandrasekaran, Jeyabharathy;Brumin, Marina;Gal-On, Amit
通讯作者：
Gal-On, Amit