Profiling of co-metabolic degradation of tetracycline by the bio-cathode in microbial fuel cells.
Profiling of co-metabolic degradation of tetracycline by the bio-cathode in microbial fuel cells.
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DOI：
10.1039/d1ra07600k
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发表时间：
2021-12-20
期刊：
影响因子：
3.9
通讯作者：
Shi Y
中科院分区：
文献类型：
--
作者：
Wang L;Liang D;Shi Y
构建了微生物燃料电池（MFC）生物阴极降解四环素（TEC）的系统。通过单因素试验研究了TEC的共代谢降解性能，并利用E.大肠杆菌生长抑制率和抗性基因。高通量测序（HTS）被用来描绘生物阴极的生物膜群落结构。结果表明，在TEC初始浓度为10 mg L−1、乙酸钠浓度为0.2-0.7 g L−1、通气量为12-18 L h−1的最佳条件下，TEC的降解率可达90%以上。与好氧生物降解TEC相比，MFC生物阴极对TEC的降解效率提高了50%，降解36 h后TEC的生态毒性降低了60.9%，出水中的TEC ARGs也有所降低。HTS结果表明，阴极生物膜中的主要优势菌为电化学活性菌醋酸杆菌属和耐TEC降解菌丝微菌属、梭菌属和红曲霉属。此外，基于5个中间体，提出了TEC的降解途径，包括脱酰胺、溴代二甲基化、脱二甲基和脱羟基。TEC在生物阴极上的降解主要是由微生物共代谢作用引起的。本研究丰富了MFC生物阴极降解水中抗生素的研究。构建了微生物燃料电池（MFC）生物阴极降解四环素（TEC）的系统。
In this paper, a system of tetracycline (TEC) degradation by the bio-cathode in a microbial fuel cell (MFC) was constructed. Overall, the co-metabolic degradation performance of TEC was studied through single factor experiments and the ecological risk was evaluated using the E. coli growth inhibition rate and resistance genes. High throughput sequencing (HTS) was utilized to profile the biofilm community structure of the bio-cathode. Results showed that the degradation rate of TEC reached greater than 90% under optimal conditions, which was 10 mg L−1 initial TEC concentration, 0.2–0.7 g L−1 sodium acetate concentration and 12–18 L h−1 aeration. Furthermore, compared with the aerobic biodegradation of TEC, the degradation efficiency of the MFC bio-cathode for TEC was significantly increased by 50% and the eco-toxicity of TEC after 36 hour degradation was reduced by 60.9%, and TEC ARGs in effluent were cut down. HTS results showed that electrochemically active bacteria Acetobacter and TEC-resistant degradation bacteria Hyphomicrobium, Clostridium and Rhodopseudomonas were the main dominant bacteria in the cathode biofilm. Besides, based on 5 intermediates, degradation pathways involving deamidation, denitro dimethylation, dedimethylation and dehydroxylation of TEC were proposed. The degradation of TEC on the bio-cathode was mainly caused by microbial co-metabolism action. This study would enrich the study of MFC bio-cathodic degradation of antibiotics in water. In this paper, a system of tetracycline (TEC) degradation by the bio-cathode in a microbial fuel cell (MFC) was constructed.
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