In vitro production of pig embryos: a point of view.
In vitro production of pig embryos: a point of view.
复制标题
猪胚胎的体外生产：一种观点。
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
R. Romar
中科院分区：
文献类型：
--
作者：
P. Coy;R. Romar
猪胚胎已成为不同生殖生物技术方案的原料，在基因转移方案和异种移植中，体外生产胚胎比体内生产胚胎具有一些优势。尽管有这样一个充满希望的未来，几个问题限制了成功的体外生产（IVP）的可行的猪胚胎。由于与不同技术相关的几个问题，猪IVP尚未完全开发，例如体外成熟后的最终成熟状态不完全，体外受精后多精受精的发生率高，培养结束时囊胚发育率低且质量差。通过研究比较体内和体外条件、精子处理技术标准化、测试培养基中的新添加剂和开发胞浆内单精子注射程序，可以改善结果。本文的第一个目的是总结关于这个问题的主要研究。其次，我们为开始研究猪胚胎IVP的研究人员提供了指南，特别提到了每种不同技术的第一篇论文和最新成就。第三，我们为未来的实验提供了建议，旨在改善每种技术的结果。
Porcine embryos have become raw materials for different programmes of reproductive biotechnology and the in vitro production of embryos has some advantages over in vivo production in gene transfer programmes and for xenotransplantation. Despite this promising future, several problems limit the success of the in vitro production (IVP) of viable porcine embryos. Porcine IVP has not been fully developed because of several problems associated with different techniques, such as incomplete final maturation status after in vitro maturation, a high incidence of polyspermy after in vitro fertilization and a low development rate and poor quality of blastocysts at the end of culture. The results could be improved with studies comparing in vivo and in vitro conditions, standardization of techniques for sperm processing, testing new additives in the culture media and developing intracytoplasmic sperm injection procedures. The first objective of the present article is to summarize the main studies published on the subject. Second, we provide a guide for researchers starting work on the IVP of pig embryos, making special mention of first papers and the most recent achievements for each of the different techniques. Third, we provide suggestions for future experiments designed to improve the results of each technique.
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