The FUSED LEAVES1-ADHERENT1 regulatory module is required for maize cuticle development and organ separation.
The FUSED LEAVES1-ADHERENT1 regulatory module is required for maize cuticle development and organ separation.
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DOI：
10.1111/nph.16837
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Gallavotti A
中科院分区：
文献类型：
--
作者：
Liu X;Bourgault R;Galli M;Strable J;Chen Z;Feng F;Dong J;Molina I;Gallavotti A
陆地植物的所有气生表皮细胞都被角质层所覆盖，角质层是一种细胞外疏水层，可以保护植物免受非生物和生物胁迫，并防止器官在发育过程中融合。将经典玉米粘附体1（ad 1）突变体的遗传和形态学分析与玉米MYB转录因子FUSED LEAVES 1（FDL 1）的全基因组结合分析结合起来，再加上fdl 1突变体的转录谱分析。我们发现，AD 1编码表皮表达的3-酮酰辅酶A合成酶（KCS），属于参与表皮蜡生物合成的功能不明的KCS酶分支。对ad 1突变体的蜡分析表明，ad 1在形成极长链蜡组分中发挥作用。我们证明，FDL 1直接结合到CCAACC核心基序存在于AD 1的调控区，以激活其表达。另外还鉴定了FDL 1的2000多个靶基因，包括许多涉及角质层形成、干旱反应和细胞壁组织的基因。我们的研究结果确定了玉米角质层生物合成的调节模块，在单子叶植物和真双子叶植物中是保守的，并强调了以前未描述的脂质代谢，运输和信号传导的因素，协调器官发育和角质层形成。
All aerial epidermal cells in land plants are covered by the cuticle, an extracellular hydrophobic layer that provides protection against abiotic and biotic stresses and prevents organ fusion during development. Genetic and morphological analysis of the classic maize adherent1 (ad1) mutant was combined with genome‐wide binding analysis of the maize MYB transcription factor FUSED LEAVES1 (FDL1), coupled with transcriptional profiling of fdl1 mutants. We show that AD1 encodes an epidermally‐expressed 3‐KETOACYL‐CoA SYNTHASE (KCS) belonging to a functionally uncharacterized clade of KCS enzymes involved in cuticular wax biosynthesis. Wax analysis in ad1 mutants indicates that AD1 functions in the formation of very‐long‐chain wax components. We demonstrate that FDL1 directly binds to CCAACC core motifs present in AD1 regulatory regions to activate its expression. Over 2000 additional target genes of FDL1, including many involved in cuticle formation, drought response and cell wall organization, were also identified. Our results identify a regulatory module of cuticle biosynthesis in maize that is conserved across monocots and eudicots, and highlight previously undescribed factors in lipid metabolism, transport and signaling that coordinate organ development and cuticle formation.
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影响因子：
7.2
作者：
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