Lipid exchange between Borrelia burgdorferi and host cells.
Lipid exchange between Borrelia burgdorferi and host cells.
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DOI：
10.1371/journal.ppat.1003109
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发表时间：
2013-01
期刊：
影响因子：
6.7
通讯作者：
Benach JL
中科院分区：
文献类型：
--
作者：
Crowley JT;Toledo AM;LaRocca TJ;Coleman JL;London E;Benach JL
伯氏疏螺旋体是莱姆病的病原体，它具有胆固醇和胆固醇-糖脂，这是细菌适应所必需的，具有抗原性，并且在介导与真核宿主细胞的相互作用中可能很重要。我们发现螺旋体可以从上皮细胞的质膜获取胆固醇。此外，通过荧光和共聚焦显微镜结合生化方法，我们证明了用荧光胆固醇类似物bodippy -胆固醇标记的伯氏疏螺旋体或3h标记的胆固醇将胆固醇和胆固醇-糖脂转移到HeLa细胞。这种转移通过两种不同的机制发生，通过细菌和真核细胞之间的直接接触和/或通过外膜囊泡的释放。因此，螺旋体和宿主细胞之间可以发生双向脂质交换。这种脂质交换可能是导致莱姆病发病的一个重要过程。莱姆病是北美最普遍的节肢动物传播疾病，由伯氏疏螺旋体引起。胆固醇是伯氏疏螺旋体膜脂的重要组成部分，并被加工成胆固醇-糖脂。我们对伯氏疏螺旋体中胆固醇存在的兴趣最近导致了螺旋体中真核样脂筏的鉴定和表征。伯氏疏螺旋体中游离胆固醇和胆固醇-糖脂的存在为真核细胞中脂筏成分的脂质相互作用创造了机会。我们提出的证据表明，在伯氏疏螺旋体和上皮细胞之间存在双向脂质交换。螺旋体不能合成胆固醇，但可以从上皮细胞的质膜获得。此外，伯氏疏螺旋体的游离胆固醇和胆固醇-糖脂通过直接接触和通过外膜囊泡转移到上皮细胞。螺旋体与宿主之间的胆固醇交换可能是莱姆病发病机制的一个重要方面。
Borrelia burgdorferi, the agent of Lyme disease, has cholesterol and cholesterol-glycolipids that are essential for bacterial fitness, are antigenic, and could be important in mediating interactions with cells of the eukaryotic host. We show that the spirochetes can acquire cholesterol from plasma membranes of epithelial cells. In addition, through fluorescent and confocal microscopy combined with biochemical approaches, we demonstrated that B. burgdorferi labeled with the fluorescent cholesterol analog BODIPY-cholesterol or 3H-labeled cholesterol transfer both cholesterol and cholesterol-glycolipids to HeLa cells. The transfer occurs through two different mechanisms, by direct contact between the bacteria and eukaryotic cell and/or through release of outer membrane vesicles. Thus, two-way lipid exchange between spirochetes and host cells can occur. This lipid exchange could be an important process that contributes to the pathogenesis of Lyme disease. Lyme disease, the most prevalent arthropod-borne disease in North America, is caused by the spirochete Borrelia burgdorferi. Cholesterol is a significant component of the B. burgdorferi membrane lipids, and is processed to make cholesterol-glycolipids. Our interest in the presence of cholesterol in B. burgdorferi recently led to the identification and characterization of eukaryotic-like lipid rafts in the spirochete. The presence of free cholesterol and cholesterol-glycolipids in B. burgdorferi creates an opportunity for lipid-lipid interactions with constituents of the lipid rafts in eukaryotic cells. We present evidence that there is a two-way exchange of lipids between B. burgdorferi and epithelial cells. Spirochetes are unable to synthesize cholesterol, but can acquire it from the plasma membrane of epithelial cells. In addition, free cholesterol and cholesterol-glycolipids from B. burgdorferi are transferred to epithelial cells through direct contact and through outer membrane vesicles. The exchange of cholesterol between spirochete and host could be an important aspect of the pathogenesis of Lyme disease.
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影响因子：
3.6
作者：
Eggers, CH;Caimano, MJ;Radolf, JD
通讯作者：
Radolf, JD
DOI：
10.1073/pnas.90.15.7059
发表时间：
1993-08-01
影响因子：
11.1
作者：
COBURN, J;LEONG, JM;ERBAN, JK
通讯作者：
ERBAN, JK
影响因子：
3.2
作者：
BELISLE, JT;BRANDT, ME;NORGARD, MV
通讯作者：
NORGARD, MV
影响因子：
3.1
作者：
COLEMAN, JL;ROGERS, RC;BENACH, JL
通讯作者：
BENACH, JL
影响因子：
64.5
作者：
Coleman, JL;Gebbia, JA;Benach, JL
通讯作者：
Benach, JL