Investigation of genetic linkage between myosin and actin genes using an interspecific mouse back-cross
Investigation of genetic linkage between myosin and actin genes using an interspecific mouse back-cross
复制标题
使用种间小鼠回交研究肌球蛋白和肌动蛋白基因之间的遗传连锁
DOI：
10.1038/314181a0
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发表时间：
1985
期刊：
影响因子：
64.8
通讯作者：
M. Buckingham
中科院分区：
文献类型：
--
作者：
B. Robert;P. Barton;A. Minty;P. Daubas;A. Weydert;F. Bonhomme;J. Catalan;Dominique Chazottes;J. Guénet;M. Buckingham
引入克隆探针来跟踪DNA限制性片段长度多态性（RFLPs）的分离，导致孟德尔遗传学在绘制人类基因组图谱方面的复兴。然而，在小鼠中，通常证明难以用DNA探针检测实验室小鼠的不同近交系之间的RFLP。为了解决这个问题，我们使用了两个物种，小家鼠和小家鼠2，它们作为同域物种3相互作用，但可以在实验室条件下杂交4。由于这些物种之间的相对进化距离，它们在更多的位点上表现出多态性，而不是通常的M。M.实验室小鼠2.当比较编码特定基因的限制性片段的大小时，在DNA水平上也观察到了这一点5。我们在M. M. Acetylticus和M. spretus跟踪这些物种之间的回交后代中编码肌球蛋白碱性轻链的不同亚型的基因的分离，并将其与其他收缩蛋白基因的分离进行比较。没有观察到这些基因之间的连锁。
The introduction of cloned probes to follow the segregation of DNA restriction fragment length polymorphisms (RFLPs) has led to a revival of mendelian genetics in attempts to map the human genome1. In the mouse, however, it has often proved difficult to detect an RFLP with a DNA probe between different inbred strains of the laboratory mouse. To circumvent this problem, we have used two species, Mus musculus domesticus and Mus spretus2 which interact as sympatric species3 but can be interbred under laboratory conditions4. Because of the relative evolutionary distance between these species, they exhibit polymorphism at many more loci than do different strains of the usual M. m. domesticus laboratory mouse2. This is also observed at the DNA level when the sizes of restriction fragments encoding a specific gene are compared5. We have used these RFLPs between M. m. domesticus and M. spretus to follow the segregation of genes encoding different isoforms of myosin alkali light chains in the backcross progeny between these species and to compare this with that of other contractile protein genes. No linkage between these genes was observed.
影响因子：
56.9
作者：
L. Leinwand;R. Fournier;B. Nadal-Ginard;T. Shows
通讯作者：
L. Leinwand;R. Fournier;B. Nadal-Ginard;T. Shows