Actin-binding Rho activating protein is expressed in the central nervous system of normal adult rats.
Actin-binding Rho activating protein is expressed in the central nervous system of normal adult rats.
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肌动蛋白结合 Rho 激活蛋白在正常成年大鼠的中枢神经系统中表达
DOI：
10.3969/j.issn.1673-5374.2012.13.001
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发表时间：
2012-05-05
影响因子：
6.1
通讯作者：
Schaper J
中科院分区：
文献类型：
--
作者：
Liu L;Luo M;Yang B;Wu X;Zhu W;Guan Y;Cai W;Troidl K;Schaper W;Schaper J
先前的研究表明，肌动蛋白结合 Rho 激活蛋白 (Abra) 在心肌细胞和血管平滑肌细胞中表达。在本研究中，我们通过共聚焦免疫荧光研究了正常成年大鼠中枢神经系统中 Abra 的表达谱。结果显示，Abra免疫染色位于中枢神经系统神经元的细胞核、细胞质和突起，其中细胞核染色最强；大脑皮质中，Abra阳性神经元体和突起分布于分子层、外颗粒层、外锥体层、内颗粒层、内锥体层和多态层6个皮质层。海马Abra阳性神经元细胞体均匀分布于锥体层和颗粒层，分子层和定向层有阳性突起；小脑皮质Abra染色显示浦肯野细胞层和颗粒层神经元细胞体阳性，分子层阳性突起；在脊髓中，Abra 免疫阳性产物覆盖了整个灰质和白质；共定位研究表明，Abra 在神经元细胞质和突起中与 F-肌动蛋白共染色，但在细胞核中的共染色较弱。此外，在海马体中，Abra 仅在神经元过程中与 F-肌动蛋白共染色，而在细胞体中则不然。该研究首次全面概述了Abra在中枢神经系统中的表达，为进一步研究Abra在成熟中枢神经系统中的作用提供了见解。
Previous studies show that actin-binding Rho activating protein (Abra) is expressed in cardiomyocytes and vascular smooth muscle cells. In this study, we investigated the expression profile of Abra in the central nervous system of normal adult rats by confocal immunofluorescence. Results showed that Abra immunostaining was located in neuronal nuclei, cytoplasm and processes in the central nervous system, with the strongest staining in the nuclei; in the cerebral cortex, Abra positive neuronal bodies and processes were distributed in six cortical layers including molecular layer, external granular layer, external pyramidal layer, internal granular layer, internal pyramidal layer and polymorphic layer; in the hippocampus, the cell bodies of Abra positive neurons were distributed evenly in pyramidal layer and granular layer, with positive processes in molecular layer and orien layer; in the cerebellar cortex, Abra staining showed the positive neuronal cell bodies in Purkinje cell layer and granular layer and positive processes in molecular layer; in the spinal cord, Abra-immunopositive products covered the whole gray matter and white matter; co-localization studies showed that Abra was co-stained with F-actin in neuronal cytoplasm and processes, but weakly in the nuclei. In addition, in the hippocampus, Abra was co-stained with F-actin only in neuronal processes, but not in the cell body. This study for the first time presents a comprehensive overview of Abra expression in the central nervous system, providing insights for further investigating the role of Abra in the mature central nervous system.
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影响因子：
1.3
作者：
Yamashiro, Shigeko
通讯作者：
Yamashiro, Shigeko
影响因子：
5.5
作者：
Cornachione, Anabelle S.;Rassier, Dilson E.
通讯作者：
Rassier, Dilson E.
影响因子：
3.7
作者：
Hu X;Kuhn JR
通讯作者：
Kuhn JR
DOI：
10.1161/atvbaha.109.195305
发表时间：
2009-12-01
影响因子：
8.7
作者：
Troidl, Kerstin;Rueding, Inka;Troidl, Christian
通讯作者：
Troidl, Christian
影响因子：
15.9
作者：
Kuwahara, Koichiro;Pipes, Gordon C. Teg;Olson, Eric N.
通讯作者：
Olson, Eric N.