Genomic characteristics of mcr-1 and bla(CTX-M-type) in a single multidrug-resistant Escherichia coli ST93 from chicken in China.
Genomic characteristics of mcr-1 and bla(CTX-M-type) in a single multidrug-resistant Escherichia coli ST93 from chicken in China.
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中国鸡多重耐药大肠杆菌 ST93 中 mcr-1 和 blaCTX-M 型的基因组特征
DOI：
10.1016/j.psj.2021.101074
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发表时间：
2021-05
期刊：
影响因子：
4.4
通讯作者：
Yuan L
中科院分区：
文献类型：
--
作者：
Li W;Li Y;Jia Y;Sun H;Zhang C;Hu G;Yuan L
本研究旨在探究中国鸡肉源一株多重耐药大肠杆菌LWY24中mcr - 1和blaCTX - M型基因的传播特征。通过接合试验、S1脉冲场凝胶电泳、Southern杂交及全基因组测序分析来确定LWY24分离株的遗传特征。同时，利用双重接合试验筛选LWY24分离株中质粒的共转移情况。LWY24分离株被鉴定为ST93型，携带3种接合性质粒，即携带blaCTX - M - 55的IncFⅡ型质粒pLWY24J - 3、携带mcr - 1的IncI2型质粒pLWY24J - mcr - 1和非耐药的IncI1型质粒pLWY24J - 4，以及一种非接合性质粒pLWY24（含blaCTX - M - 14的IncHI2/IncHI2A）。在这4种质粒中发现了众多耐药基因、插入序列（尤其是IS26）和转座子，这表明通过质粒接合、同源重组和转座发生了水平转移。在头孢噻肟和黏菌素或仅头孢噻肟的选择压力下，携带mcr - 1的质粒和携带blaCTX - M - 55的质粒能够以相似的频率共转移，每供体细胞分别产生8.00×10⁻⁴或9.00×10⁻⁴个接合子。编码mcr - 1的质粒中特定的shufflon区域可产生多达6种不同的PilV结构，这可能进一步加速质粒的水平转移。综上所述，LWY24分离株中mcr - 1和blaCTX - M型基因的传播特征可能归因于ST93的克隆传播、头孢噻肟的选择压力、IS26介导的同源重组和转座以及特定的shufflon区域。
This study was undertaken to discern the transmission characteristics of mcr-1 and blaCTX-M-type in one multidrug-resistant Escherichia coli LWY24 from chicken in China. The genetic profiles of LWY24 isolate were determined by conjugation, S1-pulsed-field gel electrophoresis, southern blot hybridization, and whole genome sequencing analysis. Meanwhile, co-transfer of plasmids in LWY24 isolate was screened by dual conjugation assays. The LWY24 isolate was identified as ST93, and harbored 3 conjugative plasmids, pLWY24J-3 (blaCTX-M-55-bearing IncFⅡ), pLWY24J-mcr-1 (mcr-1-carrying IncI2), and pLWY24J-4 (non-resistance-conferring IncI1), and one nonconjugative plasmid pLWY24 (blaCTX-M-14-containing IncHI2/IncHI2A). Numerous resistance genes, insertion sequences (especially IS26), and transposons were found in the 4 plasmids, suggesting that horizontal transmission have occurred by plasmid mating, homologous recombination, and transpositions. Under the selection pressure of cefotaxime and colistin or cefotaxime alone, the mcr-1-bearing plasmid and the blaCTX-M-55-harboring plasmid could be co-transferred at a similar frequency, with 8.00 × 10−4 or 9.00 × 10−4 transconjugants per donor cell, respectively. The specific shufflon region in mcr-1-encoding plasmid could generate up to 6 diverse PilV structures, which may further accelerate the horizontal transfer of plasmid. In conclusion, the transmission characteristics of mcr-1 and blaCTX-M-type in LWY24 isolate could due to clonal spread of ST93, selective pressure of cefotaxime, IS26-mediate homologous recombination and transposition, and the specific shufflon region.
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