Identification of rat γ atrial natriuretic polypeptide and characterization of the cDNA encoding its precursor
Identification of rat γ atrial natriuretic polypeptide and characterization of the cDNA encoding its precursor
复制标题
大鼠 γ 心房钠尿多肽的鉴定及其前体 cDNA 的表征
作者：
K. Kangawa;Y. Tawaragi;S. Oikawa;A. Mizuno;Y. Sakuragawa;H. Nakazato;A. Fukuda;N. Minamino;H. Matsuo
利尿剂和平滑肌松弛肽，命名为心房利钠肽（ANP），已在人类1 -3和大鼠4 -10心房组织中鉴定，并涉及液体容量和血管功能的控制11，12。最近，编码人2和大鼠13 -15 ANP前体的cDNA已被测序。我们先前从人体组织中分离出分子量（MW）为13，000的利钠肽（γ-hANP），包含126个氨基酸残基，是迄今为止鉴定出的最大的利钠肽，并表明其通过去除信号肽直接衍生自151个残基的人ANP前体2，3。我们现在报告一种新的大鼠心房利钠肽（γ-rANP）的分离和序列分析，分子量为13，000，它来自大鼠ANP前体。我们还报告了152个残基的大鼠ANP前体的cDNA的分子克隆和核苷酸序列分析，这是非常相似的人151个残基的前体（前hANP），除了在C-末端。大鼠和人类前体核苷酸序列在终止密码子周围的差异导致加工模式的差异。
Diuretic and smooth muscle-relaxing peptides, designated atrial natriuretic peptides (ANPs), have been identified in human1–3 and rat4–10 atrial tissues and implicated in the control of fluid volume and vascular function11,12. Recently, cDNAs encoding the human2 and rat13–15 ANP precursors have been sequenced. We previously isolated from human tissue a natriuretic peptide of molecular weight (MW) 13,000 (γ-hANP) comprising 126 amino acid residues, the largest natriuretic peptide so far identified, and showed that it is directly derived from the 151-residue human ANP precursor by the removal of a signal peptide2,3. We now report the isolation and sequence analysis of a novel rat atrial natriuretic peptide (γ-rANP) of MW 13,000, which derives from the rat ANP precursor. We also report the molecular cloning and nucleotide sequence analysis of the cDNA of the 152-residue rat ANP precursor, which is remarkably similar to the human 151-residue precursor (pre-hANP) except at the C-terminus. Differences in the rat and human precursor nucleotide sequences around the termination codons lead to a difference in processing pattern.
影响因子：
56.9
作者：
C. Seidman;A. Duby;E. Choi;R. Graham;E. Haber;C. Homcy;J. Smith;J. Seidman
通讯作者：
C. Seidman;A. Duby;E. Choi;R. Graham;E. Haber;C. Homcy;J. Smith;J. Seidman