Thrombomodulin-mediated Cell Adhesion
Thrombomodulin-mediated Cell Adhesion
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血栓调节蛋白介导的细胞粘附
作者：
Huey;G. Shi;Shinn;C. Shi;Chunping Wu;H. Yang;Hua
血栓调节蛋白（TM）是一种完整的膜糖蛋白，是一种有效的抗凝因子。TM也可能具有不同于其抗凝活性的功能。在此研究了TM对TM阴性黑色素瘤A2058细胞中细胞粘附的影响，TM阴性黑色素瘤A2058细胞转染有绿色荧光蛋白标记的TM（TMG）或凝集素结构域缺失的TM（TMG（ΔL））。共聚焦显微镜观察表明，TMG和TMG（ΔL）均分布在质膜上。TMG表达的细胞生长为紧密聚集的集落，TM显著定位于细胞间边界。TMG（ΔL）表达细胞单独生长。TMG的过表达，而不是TMG（ΔL），降低单层细胞的渗透性在体外和肿瘤生长在体内。表达TMG的细胞中细胞与细胞的粘附是Ca 2+依赖性的，并且可以被针对TM的凝集素样结构域的单克隆抗体抑制。加入甘露糖、硫酸软骨素A或硫酸软骨素C可消除TM介导的细胞粘附作用。此外，抗凝集素样结构域抗体破坏了来自正常人表皮的内源性TM表达的角质形成细胞HaCaT细胞系的紧密聚集。双标记免疫荧光染色显示类似的分布TM和肌动蛋白丝在皮层区域的TMG表达的细胞。因此，TM可以作为Ca2+依赖性细胞间粘附分子发挥作用。特异性碳水化合物与凝集素样结构域的结合对于这种特异性功能是必需的。
Thrombomodulin (TM) is an integral membrane glycoprotein that is a potent anticoagulant factor. TM may also possess functions distinct from its anticoagulant activity. Here the influence of TM on cell adhesion was studied in TM-negative melanoma A2058 cells transfected with green fluorescent protein-tagged TM (TMG) or lectin domain-deleted TM (TMG(ΔL)). Confocal microscopy demonstrated that both TMG and TMG(ΔL) were distributed in the plasma membrane. TMG-expressed cells grew as closely clustered colonies, with TM localized prominently in the intercellular boundaries. TMG(ΔL)-expressed cells grew singly. Overexpression of TMG, but not TMG(ΔL), decreased monolayer permeability in vitro and tumor growth in vivo. The cell-to-cell adhesion in TMG-expressed cells was Ca2+-dependent and was inhibited by monoclonal antibody against the lectin-like domain of TM. The effects of TM-mediated cell adhesion were abolished by the addition of mannose, chondroitin sulfate A, or chondroitin sulfate C. In addition, anti-lectin-like domain antibody disrupted the close clustering of the endogenous TM-expressed keratinocyte HaCaT cell line derived from normal human epidermis. Double-labeling immunofluorescence staining revealed similar distributions of TM and actin filament in the cortex region of the TMG-expressed cells. Thus, TM can function as a Ca2+-dependent cell-to-cell adhesion molecule. Binding of specific carbohydrates to the lectin-like domain is essential for this specific function.
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细胞接触位点的细胞质面。
影响因子：
6.8
作者：
Pokutta,Sabine;Weis,WilliamI
通讯作者：
Weis,WilliamI
P-选择素、癌转移和肝素：具有治疗意义的新机制联系。
DOI：
10.1590/s0100-879x2001000600003
发表时间：
2001
期刊：
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
影响因子：
--
作者：
Varki,A;Varki,NM
通讯作者：
Varki,NM
转基因小鼠中人血栓调节蛋白的角质形成细胞特异性表达：对表皮分化和皮肤伤口愈合的影响。
DOI：
--
发表时间：
1998
期刊：
Journal of investigative medicine : the official publication of the American Federation for Clinical Research.
影响因子：
--
作者：
Raife,TJ;Lager,DJ;Peterson,JJ;Erger,RA;Lentz,SR
通讯作者：
Lentz,SR
影响因子：
3.7
作者：
Herren, B;Garton, KJ;Raines, EW
通讯作者：
Raines, EW
DOI：
--
发表时间：
1995-04
期刊：
The American journal of pathology
影响因子：
--
作者：
Donna J. Lager;Edward J. Callaghan;Stephen F. Worth;T. Raife;Steven R. Lentzt
通讯作者：
Donna J. Lager;Edward J. Callaghan;Stephen F. Worth;T. Raife;Steven R. Lentzt