Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity.
Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity.
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DOI：
10.1093/nar/gkr1287
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发表时间：
2012-05
影响因子：
14.9
通讯作者：
Suzuki T
中科院分区：
文献类型：
--
作者：
Kimura S;Ikeuchi Y;Kitahara K;Sakaguchi Y;Suzuki T;Suzuki T
rRNA的修饰聚集在核糖体的功能区域。在大肠杆菌23 S rRNA的HST 74中，2069和2445位的鸟苷分别被修饰为7-甲基鸟苷（m7 G）和N2-甲基鸟苷（m2 G）。我们搜索了负责m7 G2069形成的基因，并鉴定了编码m2 G2445的甲基转移酶的rlmL负责m7 G2069的生物发生。rRNA的体外甲基化显示，rlmL编码负责形成m7 G2069和m2 G2445的融合甲基转移酶。我们将基因重命名为rlmKL。用于m2 G2445形成的N-末端RlmL活性被C-末端RlmK显著增强。此外，RlmKL具有H7 G74的解旋活性，在50 S亚基的生物发生过程中促进m7 G2069和m2 G2445的协同甲基化。事实上，我们观察到RlmKL参与了缺乏RNA解旋酶deaD的突变株中50 S亚基的有效组装。
Modifications of rRNAs are clustered in functional regions of the ribosome. In Helix 74 of Escherichia coli 23S rRNA, guanosines at positions 2069 and 2445 are modified to 7-methylguanosine(m7G) and N2-methylguanosine(m2G), respectively. We searched for the gene responsible for m7G2069 formation, and identified rlmL, which encodes the methyltransferase for m2G2445, as responsible for the biogenesis of m7G2069. In vitro methylation of rRNA revealed that rlmL encodes a fused methyltransferase responsible for forming both m7G2069 and m2G2445. We renamed the gene rlmKL. The N-terminal RlmL activity for m2G2445 formation was significantly enhanced by the C-terminal RlmK. Moreover, RlmKL had an unwinding activity of Helix 74, facilitating cooperative methylations of m7G2069 and m2G2445 during biogenesis of 50S subunit. In fact, we observed that RlmKL was involved in the efficient assembly of 50S subunit in a mutant strain lacking an RNA helicase deaD.
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影响因子：
11.4
作者：
Ikeuchi, Yoshiho;Kitahara, Kei;Suzuki, Tsutomu
通讯作者：
Suzuki, Tsutomu
影响因子：
2.9
作者：
Bizebard, T;Ferlenghi, I;Dreyfus, M
通讯作者：
Dreyfus, M
大肠杆菌K-12的构造框架，单基因敲除突变体：Keio Collection。
影响因子：
9.9
作者：
通讯作者：
--
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
11.4
作者：
Das, Gautam;Thotala, Dinesh Kumar;Varshney, Umesh
通讯作者：
Varshney, Umesh