Regulation of Na+, K(+)-ATPase in the rat outer medullary collecting duct during potassium depletion.
Regulation of Na+, K(+)-ATPase in the rat outer medullary collecting duct during potassium depletion.
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缺钾期间大鼠外髓集合管中 Na , K( )-ATP 酶的调节。
DOI：
--
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发表时间：
1998
影响因子：
13.6
通讯作者：
A. Doucet
中科院分区：
文献类型：
--
作者：
B. Buffin;J. Verbavatz;L. Cheval;S. Marsy;M. Younes;C. Le Moal;A. Doucet
在K(+)缺乏大鼠的外髓集合管(OMCD)中，K+的分泌被抑制，而激活这种分泌的Na+，K(+)-ATPase被显著地激活，因此认为Na+，K(+)-ATPase错位到顶端细胞膜上，使K+重新吸收。与正常大鼠相比，哇巴因在K(+)耗竭大鼠中的矛盾效应支持了这一假说。然而，我们最近发现哇巴因不仅抑制K(+)缺乏大鼠OMCD的Na+，K(+)-ATPase，而且还抑制顶端H+，K(+)-ATPase。因此，本研究旨在评估以前的观察结果是由Na+，K(+)-ATPase还是哇巴因敏感的H+，K(+)-ATPase解释的。Na+，K(+)-ATPase与H+，K(+)-ATPase的区别在于它对SCH-28080不敏感。结果表明，饲喂K(+)缺乏2wk的大鼠OMCD中，Na+，K(+)-ATPase的水解性和转运活性、功能单位的数量及其α1和β1亚基的mRNA表达增加了3倍以上。免疫荧光显示，K(+)缺乏大鼠的Na+，K(+)-ATPase染色明显增强，但仍定位于OMCD主细胞的基底外侧极。总之，K+耗竭与大鼠OMCD基底外侧极功能性Na+，K+泵的显著诱导有关。因此，K+分泌减少可能是由于抑制心尖部K+电导和刺激基底侧K+循环所致。提示Na+，K(+)-ATPase升高参与了K(+)缺乏大鼠集合管Na+重吸收的增加。
Because in outer medullary collecting ducts (OMCD) of K(+)-depleted rats, K+ secretion is abolished, whereas Na+, K(+)-ATPase, which energizes this secretion, is markedly stimulated, it has been proposed that Na+, K(+)-ATPase was mislocated to the apical cell membrane and energized K+ reabsorption. This hypothesis has been supported by paradoxical effects of ouabain in K(+)-depleted compared with normal rats. However, we have recently shown that ouabain inhibits not only Na+, K(+)-ATPase but also apical H+, K(+)-ATPase in the OMCD of K(+)-depleted rats. Therefore, this study was designed to evaluate whether previous observations were accounted for by Na+, K(+)-ATPase or by ouabain-sensitive H+, K(+)-ATPase. Na+, K(+)-ATPase was distinguished from H+, K(+)-ATPase by its insensitivity to Sch-28080. Results indicate that the hydrolytic and transport activities of Na+, K(+)-ATPase, the number of its functional units, and the expression of mRNA of its alpha 1 and beta 1 subunits were increased threefold or more in the OMCD of rats fed a K(+)-depleted diet for 2 wk. By immunofluorescence, Na+, K(+)-ATPase staining was strongly increased in K(+)-depleted rats but remained localized to the basolateral pole of OMCD principal cells. In conclusion, K+ depletion is associated with marked induction of functional Na+, K+ pumps at the basolateral pole of rat OMCD. Therefore, reduced K+ secretion might result from inhibition of apical K+ conductances and stimulation of basolateral K+ recycling. It is proposed that increased Na+, K(+)-ATPase participates in the increased Na+ reabsorption prevailing in collecting ducts of K(+)-depleted rats.
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DOI：
10.1152/ajprenal.1987.252.3.f447
发表时间：
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期刊：
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影响因子：
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DOI：
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DOI：
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DOI：
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DOI：
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发表时间：
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影响因子：
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