Inhibitory effect of norditerpenes on LPS-induced TNF-alpha production from the Okinawan soft coral, Sinularia sp.
Inhibitory effect of norditerpenes on LPS-induced TNF-alpha production from the Okinawan soft coral, Sinularia sp.
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去甲二萜对冲绳软珊瑚 (Sinularia sp.) LPS 诱导的 TNF-α 产生的抑制作用。
DOI：
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发表时间：
2003
影响因子：
2
通讯作者：
T. Miyamoto
中科院分区：
文献类型：
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作者：
H. Takaki;Ryoji Koganemaru;You Iwakawa;R. Higuchi;T. Miyamoto
在冲绳采集的软珊瑚（Sinularia sp.）的乙醇（EtOH）提取物对小鼠巨噬细胞样细胞（RAW264.7）产生脂多糖（LPS）诱导的肿瘤坏死因子- α (tnf - α)具有有效的抑制作用。通过活性引导纯化，分离出两种去甲二萜：去甲樟脑内酯(1)和去甲樟脑内酯(2)。这些结构是由光谱数据确定的。去甲樟脑内酯(1)和辛乐内酯(2)呈剂量依赖性抑制tnf - α的产生，且在33微克/毫升浓度下比强的松龙更有效。它们还表现出不受细胞毒性影响的对一氧化氮（NO）产生的抑制作用。
An ethanol (EtOH) extract of the soft coral (Sinularia sp.), collected in Okinawa, demonstrated a potent inhibitory effect on lipopolysaccharide (LPS)-induced tumor necrosis factor-alpha (TNF-alpha) production by murine macrophage-like cells (RAW264.7). The activity-guided purification of the EtOH extract resulted in the isolation of two norditerpenes, norcembrenolide (1) and sinuleptolide (2). These structures were identified from the spectroscopic data. Norcembrenolide (1) and sinuleptolide (2) inhibited TNF-alpha production in a dose-dependent manner, and showed a more potent effect than prednisolone at the concentration of 33 microg/ml. They also exhibited an inhibitory effect on nitric oxide (NO) production not influenced by cytotoxicity.
影响因子：
5.1
作者：
ElSayed, KA;Hamann, MT
通讯作者：
Hamann, MT