Quantifying the correlation between spatially defined oxygen gradients and cell fate in an engineered three-dimensional culture model.
Quantifying the correlation between spatially defined oxygen gradients and cell fate in an engineered three-dimensional culture model.
复制标题
量化工程三维培养模型中空间定义的氧梯度与细胞命运之间的相关性。
DOI：
10.1098/rsif.2014.0501
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发表时间：
2014-09-06
期刊：
影响因子：
--
通讯作者：
Shipley RJ
中科院分区：
文献类型：
--
作者：
Ardakani AG;Cheema U;Brown RA;Shipley RJ
三维组织培养中的挑战仍然是缺乏将营养输送和细胞分布联系起来的定量信息。在体内和体外，氧气都是通过从其来源（血管或结构边缘）扩散来输送的。氧水平在一个确定的距离从它的来源取决于扩散和细胞代谢的平衡至关重要。细胞可以通过增殖、死亡和趋化性对这种氧环境作出反应，从而导致细胞密度的空间分辨梯度。本研究提取了三维螺旋状细胞胶原蛋白结构中组织氧合、细胞增殖、活力和趋化性的空间分辨和同步数据。氧浓度梯度在高氧区驱动优先的细胞增殖速率和活力，并诱导沿着胶原蛋白结构的螺旋的趋化性;螺旋方向上1.03 mmHg mm−1的氧梯度诱导1015 μm day−1的平均迁移速度。虽然这种运动是适度的，但它有效地平衡了系统以获得稳定的细胞密度分布，并提供了对天然细胞机制的深入了解，以使细胞数量和活性适应普遍的氧气状况。
A challenge in three-dimensional tissue culture remains the lack of quantitative information linking nutrient delivery and cellular distribution. Both in vivo and in vitro, oxygen is delivered by diffusion from its source (blood vessel or the construct margins). The oxygen level at a defined distance from its source depends critically on the balance of diffusion and cellular metabolism. Cells may respond to this oxygen environment through proliferation, death and chemotaxis, resulting in spatially resolved gradients in cellular density. This study extracts novel spatially resolved and simultaneous data on tissue oxygenation, cellular proliferation, viability and chemotaxis in three-dimensional spiralled, cellular collagen constructs. Oxygen concentration gradients drove preferential cellular proliferation rates and viability in the higher oxygen zones and induced chemotaxis along the spiral of the collagen construct; an oxygen gradient of 1.03 mmHg mm−1 in the spiral direction induced a mean migratory speed of 1015 μm day−1. Although this movement was modest, it was effective in balancing the system to a stable cell density distribution, and provided insights into the natural cell mechanism for adapting cell number and activity to a prevailing oxygen regime.
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影响因子：
--
作者：
Griffith, CK;Miller, C;George, SC
通讯作者：
George, SC
影响因子：
3.8
作者：
Shipley, R. J.;Davidson, A. J.;Chan, K.;Chaudhuri, J. B.;Waters, S. L.;Ellis, M. J.
通讯作者：
Ellis, M. J.
影响因子：
3.3
作者：
Engelhardt, E. -M.;Stegberg, E.;Frey, P.
通讯作者：
Frey, P.
影响因子：
3.3
作者：
Hadjipanayi, E.;Mudera, V.;Brown, R. A.
通讯作者：
Brown, R. A.
影响因子：
46.9
作者：
Jeon, NL;Baskaran, H;Toner, M
通讯作者：
Toner, M