Circadian and ultradian rhythms of free glucocorticoid hormone are highly synchronized between the blood, the subcutaneous tissue, and the brain.
Circadian and ultradian rhythms of free glucocorticoid hormone are highly synchronized between the blood, the subcutaneous tissue, and the brain.
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DOI：
10.1210/en.2012-1484
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发表时间：
2012-09
期刊：
影响因子：
4.8
通讯作者：
Linthorst AC
中科院分区：
文献类型：
--
作者：
Qian X;Droste SK;Lightman SL;Reul JM;Linthorst AC
包括人类在内的各种物种的血浆中的总糖皮质激素水平遵循由一系列潜在的激素脉冲组成的昼夜节律。在血液中，大部分糖皮质激素与皮质类固醇结合球蛋白和白蛋白结合，导致游离激素水平降低。虽然只有游离部分具有生物活性，但令人惊讶的是，人们对游离糖皮质激素的节律知之甚少。我们使用单探针微透析直接测量自由行为大鼠血液中的游离皮质酮水平。血液中的游离皮质酮显示出明显的昼夜节律和超昼夜节律，脉冲频率约为每小时一个脉冲，同时激素水平和脉冲高度朝向亮/暗周期的活跃期增加。在皮下组织中也明显存在类似的节律，表明游离皮质酮节律从血液转移到外周靶组织。此外，在双探针微透析研究中，我们证明了血液和皮下组织中游离皮质酮的昼夜节律和超昼夜节律是高度同步的。此外，自由皮质酮的节奏也同步之间的血液和海马。这些数据首次证明了血液中游离皮质酮的超昼夜节律，其转化为外周和中央组织中游离糖皮质激素的同步节律。维持超日节律跨越外周和大脑中的组织屏障对于研究疾病中的异常生物节律和开发糖皮质激素治疗的改进方案具有重要意义。
Total glucocorticoid hormone levels in plasma of various species, including humans, follow a circadian rhythm that is made up from an underlying series of hormone pulses. In blood most of the glucocorticoid is bound to corticosteroid-binding globulin and albumin, resulting in low levels of free hormone. Although only the free fraction is biologically active, surprisingly little is known about the rhythms of free glucocorticoid hormones. We used single-probe microdialysis to measure directly the free corticosterone levels in the blood of freely behaving rats. Free corticosterone in the blood shows a distinct circadian and ultradian rhythm with a pulse frequency of approximately one pulse per hour together with an increase in hormone levels and pulse height toward the active phase of the light/dark cycle. Similar rhythms were also evident in the subcutaneous tissue, demonstrating that free corticosterone rhythms are transferred from the blood into peripheral target tissues. Furthermore, in a dual-probe microdialysis study, we demonstrated that the circadian and ultradian rhythms of free corticosterone in the blood and the subcutaneous tissue were highly synchronized. Moreover, free corticosterone rhythms were also synchronous between the blood and the hippocampus. These data demonstrate for the first time an ultradian rhythm of free corticosterone in the blood that translates into synchronized rhythms of free glucocorticoid hormone in peripheral and central tissues. The maintenance of ultradian rhythms across tissue barriers in both the periphery and the brain has important implications for research into aberrant biological rhythms in disease and for the development of improved protocols for glucocorticoid therapy.
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DOI：
10.1098/rspb.2009.2148
发表时间：
2010-06-07
期刊：
Proceedings. Biological sciences
影响因子：
--
作者：
Walker JJ;Terry JR;Lightman SL
通讯作者：
Lightman SL
影响因子：
21.3
作者：
Stavreva DA;Wiench M;John S;Conway-Campbell BL;McKenna MA;Pooley JR;Johnson TA;Voss TC;Lightman SL;Hager GL
通讯作者：
Hager GL
影响因子：
4.8
作者：
Karssen, AM;Meijer, OC;de Kloet, ER
通讯作者：
de Kloet, ER
影响因子：
4.8
作者：
KANEKO, M;KANEKO, K;DALLMAN, MF
通讯作者：
DALLMAN, MF
影响因子：
10.6
作者：
KAHN, JP;RUBINOW, DR;POST, RM
通讯作者：
POST, RM