An Acoustic Device for Ultra High-Speed Quantification of Cell Strain During Cell-Microbubble Interaction.
An Acoustic Device for Ultra High-Speed Quantification of Cell Strain During Cell-Microbubble Interaction.
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DOI：
10.1021/acsbiomaterials.3c00757
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发表时间：
2023-10-09
影响因子：
5.8
通讯作者：
Carugo, Dario
中科院分区：
文献类型：
--
作者：
Pattinson, Oliver;Keller, Sara B.;Evans, Nicholas D.;Pierron, Fabrice;Carugo, Dario
关键词：
微泡利用超声刺激下的高频振荡在细胞中诱导一系列治疗效果，通常通过机械刺激和细胞的透化。该领域中存在的最大挑战之一是在治疗相关频率下表征细胞和微泡之间的相互作用。技术限制，例如采用足够的帧速率和获得足够的图像分辨率，限制了细胞对振荡微泡的机械响应的量化。在这里，开发了一种新的方法来解决这些限制中的许多限制，并提高在高帧速率下细胞-微泡相互作用的图像分辨率。设计了一种紧凑的声学装置，用于容纳细胞和微泡以及治疗相关的声场，同时与Shimadzu HPV-X相机兼容。细胞活力测试证实了细胞的成功培养和增殖，并定量了DSPC-和阳离子DSEPC-微泡与骨肉瘤细胞的附着。在500万FPS的帧速率下在装置内观察到微泡振荡，证实了合适的声场产生和超高速图像捕获。这些图像的高空间分辨率揭示了微泡振荡后细胞中可观察到的变形，并支持首次使用数字图像相关性在单个细胞中进行应变定量。这种新型声学装置提供了一种简单有效的方法，用于提高细胞-微泡相互作用图像的空间分辨率，从而有机会了解超声暴露后驱动振荡微泡治疗效果的机制。
Microbubbles utilize high-frequency oscillations under ultrasound stimulation to induce a range of therapeutic effects in cells, often through mechanical stimulation and permeabilization of cells. One of the largest challenges remaining in the field is the characterization of interactions between cells and microbubbles at therapeutically relevant frequencies. Technical limitations, such as employing sufficient frame rates and obtaining sufficient image resolution, restrict the quantification of the cell’s mechanical response to oscillating microbubbles. Here, a novel methodology was developed to address many of these limitations and improve the image resolution of cell–microbubble interactions at high frame rates. A compact acoustic device was designed to house cells and microbubbles as well as a therapeutically relevant acoustic field while being compatible with a Shimadzu HPV-X camera. Cell viability tests confirmed the successful culture and proliferation of cells, and the attachment of DSPC- and cationic DSEPC-microbubbles to osteosarcoma cells was quantified. Microbubble oscillation was observed within the device at a frame rate of 5 million FPS, confirming suitable acoustic field generation and ultra high-speed image capture. High spatial resolution in these images revealed observable deformation in cells following microbubble oscillation and supported the first use of digital image correlation for strain quantification in a single cell. The novel acoustic device provided a simple, effective method for improving the spatial resolution of cell–microbubble interaction images, presenting the opportunity to develop an understanding of the mechanisms driving the therapeutic effects of oscillating microbubbles upon ultrasound exposure.
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影响因子：
8
作者：
Fröhlich E
通讯作者：
Fröhlich E
影响因子：
10.8
作者：
De Cock, Ine;Zagato, Elisa;Lentacker, Ine
通讯作者：
Lentacker, Ine
影响因子：
2.4
作者：
Caskey, Charles F.;Stieger, Susanne M.;Ferrara, Katherine W.
通讯作者：
Ferrara, Katherine W.
影响因子：
2.9
作者：
Couture, Olivier;Bannouf, Souad;Tanter, Mickael
通讯作者：
Tanter, Mickael
DOI：
10.1109/58.741536
发表时间：
1999-01-01
影响因子：
3.6
作者：
Dayton, PA;Morgan, KE;Ferrara, KW
通讯作者：
Ferrara, KW