Food allergy herbal formula 2 protection against peanut anaphylactic reaction is via inhibition of mast cells and basophils.
Food allergy herbal formula 2 protection against peanut anaphylactic reaction is via inhibition of mast cells and basophils.
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DOI：
10.1016/j.jaci.2010.09.013
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发表时间：
2010-12
影响因子：
14.2
通讯作者：
Li, Xiu-Min
中科院分区：
文献类型：
--
作者：
Song, Ying;Qu, Chunfeng;Srivastava, Kamal;Yang, Nan;Busse, Paula;Zhao, Wei;Li, Xiu-Min
肥大细胞和嗜碱性细胞是ige介导的过敏反应的关键效应细胞。中草药配方faf -2可以防止小鼠的花生过敏反应。然而，这种效应背后的机制尚未完全阐明。探讨FAHF-2是否抑制肥大细胞/嗜碱性粒细胞数量和IgE介导的活化。花生过敏小鼠（PNA小鼠）灌胃（ig） FAHF-2治疗7周，并在治疗后1天和4周分别用花生刺激（ig）。测定外周血嗜碱性粒细胞数目、腹膜肥大细胞数目及FcεRI的表达。研究了FAHF-2对小鼠肥大细胞系MC/9的直接作用，以及从FAHF-2中分离的4个组分和3个化合物对大鼠嗜碱性白血病细胞（RBL-2H3）和人皮肤肥大细胞脱颗粒的影响，以及对IgE介导的Syk信号通路的影响。虽然所有假药治疗的PNA小鼠都发生了过敏反应，但在治疗后1天和4周，经fahf -2治疗的PNA小鼠在花生刺激后没有发生过敏反应。治疗1周后开始降低外周血嗜碱性粒细胞，并持续治疗后至少4周。治疗4周后腹腔肥大细胞数量及fc - ε - ri表达均显著降低。FAHF-2处理的MC/9细胞显著降低了ige诱导的FcεRI表达、FcεRI γ mRNA亚基表达、增殖和组胺释放。来自FAHF-2的分数2 （F2）抑制RBL-2H3细胞和人肥大细胞脱颗粒。F2-小檗碱、棕榈碱和麻草酸三种化合物通过抑制Syk磷酸化抑制rbr - 2h3细胞脱粒。FAHF-2减少嗜碱性粒细胞和肥大细胞数量以及抑制ige介导的肥大细胞活化可能有助于FAHF-2对花生过敏反应的持续保护。FAHF-2阻断花生过敏反应。这种作用与肥大细胞/嗜碱性粒细胞活化和增殖的减少有关。FAHF-2可能是一种新的抗花生过敏反应疗法。
Mast cells and basophils are key effector cells of IgE-mediated anaphylactic reactions. The Chinese herbal formula, FAHF-2, protects against peanut anaphylaxis in mice. However, the mechanisms underlying this effect are not fully elucidated. To investigate whether FAHF-2 inhibits mast cell/basophil numbers and IgE mediated activation. Peanut allergic mice (PNA mice) were treated with FAHF-2 intragastrically (i.g.) for 7 weeks and challenged (i.g.) with peanut 1 day and 4 weeks post treatment. Peripheral blood basophil numbers and peritoneal mast cell numbers and FcεRI expression were determined. Direct effects of FAHF-2 on murine mast cell line MC/9, and effects of four fractions and three compounds isolated from FAHF-2 on rat basophilic leukemia cells (RBL-2H3) and human skin mast cells degranulation, and on the IgE mediated Syk signaling pathway were determined. While all sham-treated PNA mice developed anaphylaxis, FAHF-2-treated PNA mice were protected against anaphylaxis following peanut challenge at 1 day and 4 weeks post therapy. Reduction of peripheral blood basophils began after 1 week of treatment and continued for at least 4 weeks post therapy. The number and FcεRI expression of peritoneal mast cells were also significantly decreased 4 weeks post therapy. FAHF-2 treated MC/9 cells showed significantly reduced IgE-induced FcεRI expression, FcεRI γ mRNA subunit expression, proliferation, and histamine release upon challenge. Fraction 2 (F2) from FAHF-2 inhibited RBL-2H3 cell and human mast cell degranulation. Three compounds from F2- berberine, palmatine and jatrorrhizine inhibited of RBL-2H3 cell degranulation via suppressing Syk phosphorylation. FAHF-2 reduction of basophils and mast cell numbers as well as suppression of IgE-mediated mast cell activation may contribute to FAHF-2 persistent protection against peanut anaphylaxis. FAHF-2 blocked peanut anaphylaxis. This effect was associated with reduction of mast cells/basophils activation and proliferation. FAHF-2 may be a novel anti-peanut anaphylaxis therapy.
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DOI：
10.1111/j.1365-2222.2008.03154.x
发表时间：
2009-03
期刊：
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
影响因子：
--
作者：
Torrero MN;Larson D;Hübner MP;Mitre E
通讯作者：
Mitre E
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
4.3
作者：
Han, Eun Hee;Park, Jin Hee;Jeong, Hye Gwang
通讯作者：
Jeong, Hye Gwang
影响因子：
4.4
作者：
Kitaura, J;Xiao, WB;Kawakami, T
通讯作者：
Kawakami, T
影响因子：
4.3
作者：
Caldas, ED;Machado, LL
通讯作者：
Machado, LL