The genome and transcriptome of the enteric parasite Entamoeba invadens, a model for encystation.
The genome and transcriptome of the enteric parasite Entamoeba invadens, a model for encystation.
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DOI：
10.1186/gb-2013-14-7-r77
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发表时间：
2013-07-26
期刊：
影响因子：
12.3
通讯作者：
Singh U
中科院分区：
文献类型：
--
作者：
Ehrenkaufer GM;Weedall GD;Williams D;Lorenzi HA;Caler E;Hall N;Singh U
几种真核寄生虫形成传播感染的包囊。该过程存在于弓形虫、贾第鞭毛虫和线虫等多种生物体中。在溶组织内阿米巴中，这一过程无法在体外诱导，因此难以研究。在内阿米巴入侵中，可以诱导阶段转换，但其作为研究发育生物学的模型系统的实用性由于缺乏基因组资源而受到限制。我们对入侵大肠杆菌进行了基因组和转录组测序，以确定参与阶段转换的分子过程。我们报告了入侵大肠杆菌基因组的测序和组装，并使用全转录组测序来表征成囊和脱胞过程中基因表达的变化。侵入肠球菌的基因组比溶组织内阿米巴的基因组大，这显然主要是由于基因间区域的扩展；总体基因数量和基因调控机制在物种之间是保守的。超过一半的基因在形态形式之间的转换过程中受到调节，并且关键信号分子磷脂酶 D 似乎可以调节包囊。我们提供了成囊过程中减数分裂发生的证据，表明阶段转换可能在菌株之间的重组中发挥关键作用。我们的分析表明，远亲寄生虫之间的成囊过程有许多共同的核心过程，包括减数分裂、脂质信号传导和 RNA 修饰。这些数据为了解重要的人类病原体溶组织内阿米巴的发育级联奠定了基础，并强调了与肠道病原体更广泛相关的保守过程。
Several eukaryotic parasites form cysts that transmit infection. The process is found in diverse organisms such as Toxoplasma, Giardia, and nematodes. In Entamoeba histolytica this process cannot be induced in vitro, making it difficult to study. In Entamoeba invadens, stage conversion can be induced, but its utility as a model system to study developmental biology has been limited by a lack of genomic resources. We carried out genome and transcriptome sequencing of E. invadens to identify molecular processes involved in stage conversion. We report the sequencing and assembly of the E. invadens genome and use whole transcriptome sequencing to characterize changes in gene expression during encystation and excystation. The E. invadens genome is larger than that of E. histolytica, apparently largely due to expansion of intergenic regions; overall gene number and the machinery for gene regulation are conserved between the species. Over half the genes are regulated during the switch between morphological forms and a key signaling molecule, phospholipase D, appears to regulate encystation. We provide evidence for the occurrence of meiosis during encystation, suggesting that stage conversion may play a key role in recombination between strains. Our analysis demonstrates that a number of core processes are common to encystation between distantly related parasites, including meiosis, lipid signaling and RNA modification. These data provide a foundation for understanding the developmental cascade in the important human pathogen E. histolytica and highlight conserved processes more widely relevant in enteric pathogens.
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