RECENT DEVELOPMENTS IN FREQUENCY-DOMAIN FLUOROMETRY.
RECENT DEVELOPMENTS IN FREQUENCY-DOMAIN FLUOROMETRY.
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频域荧光测定的最新进展。
DOI：
10.1080/10739148508543578
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发表时间：
1985
影响因子：
1.6
通讯作者：
E. Gratton
中科院分区：
文献类型：
--
作者：
J. Lakowicz;B. Maliwal;E. Gratton
I.相位调制荧光法是时间分辨荧光光谱法的频域模拟。在过去的三年中，我们见证了调制频率从1到220 MHz的变频相位调制荧光计的发展。这些仪器提供了令人印象深刻的多或非指数荧光衰减的分辨率。为了介绍这些仪器，我们描述了它们的设计和操作原理。为了说明可获得的分辨率，我们提出的两个和三个组件的荧光团的混合物的分辨率，复杂的各向异性衰减的分辨率从非球形分子和时间分辨的发射光谱的测定中存在的时间依赖性的光谱弛豫的结果。目前，频域荧光计获得的分辨率似乎至少相当于单光子计数的脉冲锁模激光源获得的分辨率。这些锁模光源也可用于相位调制。
I. SUMMARY Phase-modulation fluorometry is the frequency-domain analogue of time-resolved fluorescence spectroscopy. During the past three years we witnessed the development of variable-frequency phase-modulation fluorometers with modulation frequencies from 1 to 220 MHz. These instruments provide impressive resolution of multi or non-exponential fluorescence decays. To introduce these instruments we describe their design and operational principles. To illustrate the obtainable resolution we present results for the resolution of two and three-component mixtures of fluorophores, the resolution of complex anisotropy decays from non-spherical molecules and the determination of time-resolved emission spectra in the presence of time-dependent spectral relaxation. At present, the resolution obtainable with the frequency-domain fluorometers appears to be at least equivalent to that obtained with pulsed mode-locked laser sources with single photon counting. These mode-locked sources can also be used for phase flu...
阻碍脂质双层中苝的去极化旋转。
影响因子：
2.9
作者：
Lakowicz,JR;Knutson,JR
通讯作者：
Knutson,JR
通过多频相位调制荧光测定法获得溶剂和脂质双层中二苯基己三烯和苝的时间分辨荧光各向异性。
影响因子：
2.9
作者：
Lakowicz,JR;Cherek,H;Maliwal,BP
通讯作者：
Maliwal,BP