Polymerization and matrix physical properties as important design considerations for soluble collagen formulations.
Polymerization and matrix physical properties as important design considerations for soluble collagen formulations.
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聚合和基质物理特性是可溶性胶原蛋白配方的重要设计注意事项。
DOI：
10.1002/bip.21431
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发表时间：
2010-08
期刊：
影响因子：
2.9
通讯作者：
Voytik-Harbin, S. L.
中科院分区：
文献类型：
--
作者：
Kreger, S. T.;Bell, B. J.;Bailey, J.;Stites, E.;Kuske, J.;Waisner, B.;Voytik-Harbin, S. L.
关键词：
尽管I型胶原广泛用于研究和医学应用，但其原纤维形成或聚合潜力尚未完全确定和开发。在这里，我们描述了一种I型胶原制剂，其是从猪皮（PSC）中酸溶解的，基于聚合潜力进行质量控制，并且非常适合作为用于制备三维（3D）培养系统和可注射/可植入体内细胞微环境的平台聚合物，其中相关的生物化学和生物物理参数都可以精确控制。PSC与3种商业胶原在组成和纯度以及聚合潜力方面进行了比较，所述聚合潜力通过动力学参数和原纤维微观结构以及形成的基质的机械性能来描述。当进行相同的聚合条件下，PSC显示出显着减少的聚合时间相比，其他胶原蛋白和产生的基质具有最大的机械完整性和最广泛的机械性能，其特征在于在振荡剪切，单轴拉伸，和无侧限压缩。组成和特性粘度分析表明，PSC的聚合潜力增强可能归因于其独特的低聚物组成。总的来说，这项工作证明了标准化的下一代胶原蛋白配方的聚合潜力的基础上，并提供了初步的洞察低聚物的胶原蛋白聚合性能的贡献。
Despite extensive use of type I collagen for research and medical applications, its fibril-forming or polymerization potential has yet to be fully defined and exploited. Here we describe a type I collagen formulation that is acid solubilized from porcine skin (PSC), quality controlled based upon polymerization potential, and well suited as a platform polymer for preparing three-dimensional (3D) culture systems and injectable/implantable in-vivo cellular microenvironments in which both relevant biochemical and biophysical parameters can be precision-controlled. PSC is compared with 3 commercial collagens in terms of composition and purity as well as polymerization potential, which is described by kinetic parameters and fibril microstructure and mechanical properties of formed matrices. When subjected to identical polymerization conditions, PSC showed significantly decreased polymerization times compared to the other collagens and yielded matrices with the greatest mechanical integrity and broadest range of mechanical properties as characterized in oscillatory shear, uniaxial extension, and unconfined compression. Compositional and intrinsic viscosity analyses suggest that the enhanced polymerization potential of PSC may be attributed to its unique oligomer composition. Collectively, this work demonstrates the importance of standardizing next generation collagen formulations based upon polymerization potential and provides preliminary insight into the contribution of oligomers to collagen polymerization properties.
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