Alteration of circulating mitochondrial DNA concentration after irradiation.
Alteration of circulating mitochondrial DNA concentration after irradiation.
复制标题
辐射后循环线粒体 DNA 浓度的变化。
DOI：
10.1007/978-1-4614-4989-8_52
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发表时间：
2013
影响因子：
--
通讯作者：
Okunieff,Paul
中科院分区：
文献类型：
--
作者：
Zhang,Mei;Zhang,Bingrong;Guo,Yansong;Zhang,Lei;Yang,Shanmin;Yin,Liangjie;Vidyasagar,Sadasivan;Maguire,David;Swarts,Steve;Zhang,Zhenhuan;Zhang,Amy;Zhang,Lurong;Okunieff,Paul
线粒体DNA （mtDNA）是母系遗传的，并控制与氧相关的腺苷-5 ' -三磷酸的产生，腺苷-5 ' -三磷酸从线粒体运输到其他细胞室，并作为细胞活动的能量。mtDNA与核DNA （nDNA）在物理上是分离的。电离辐射（IR）导致mtDNA和nDNA释放到循环中。我们之前的研究表明，nDNA有潜力成为一种生物剂量计。本研究采用支链DNA技术探讨IR后mtDNA的改变模式。C57BL/6小鼠分别接受0、1.5、3、6、8或10 Gy的全身辐照；之后，在3、6、9、15、24、48、72或168小时采集血浆mtDNA样本进行评估。我们发现：(1)设计的问题集对从肝脏中提取的mtDNA是特异性的，它们识别出混合在nDNA中的少量mtDNA；(2)血浆mtDNA仅在8 Gy辐照后6 h出现显著升高。mtDNA的改变不受剂量或时间的影响；因此，它不太可能是一种有效的生物剂量计。
Mitochondrial DNA (mtDNA) is maternally inherited and controls the oxygen-related production of adenosine-5′-triphosphate, which is transported from the mitochondria to other cellular compartments and used as energy for cellular activities. The mtDNA is physically separated from nuclear DNA (nDNA). Ionizing radiation (IR) causes the release of both mtDNA and nDNA into circulation. Our previous study demonstrated that nDNA has potential to be a biodosimeter. In this study, branched DNA technology was used to explore the alteration pattern of mtDNA after IR. C57BL/6 mice were exposed to 0, 1.5, 3, 6, 8, or 10 Gy total body irradiation; thereafter, plasma mtDNA was assessed with samples collected at 3, 6, 9, 15, 24, 48, 72, or 168 h. We found that: (1) the designed probesets were specific for mtDNA extracted from the liver, and they recognized the small amount of mtDNA mixed in the nDNA; (2) plasma mtDNA exhibited a statistically significant increase only at 6 h after 8 Gy irradiation. The alteration of mtDNA was not dose-dependent or time-dependent; hence, it is unlikely to be an effective biodosimeter.
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影响因子：
3.1
作者：
Flynn, Daniel F.;Goans, Ronald E.
通讯作者：
Goans, Ronald E.
DOI：
10.1016/s0360-3016(98)00185-0
发表时间：
1998-08-01
影响因子：
7
作者：
Morales, A;Miranda, M;Fernández-Checa, JC
通讯作者：
Fernández-Checa, JC
DOI：
10.1259/bjr/49886569
发表时间：
2010-08
期刊：
The British journal of radiology
影响因子：
--
作者：
L. Zhang;M. Zhang;S. Yang;Y. Cao;S. Bingrong Zhang;L. Yin;Y. Tian;Y. Ma;A. Zhang;P. Okunieff;L. Zhang
通讯作者：
L. Zhang;M. Zhang;S. Yang;Y. Cao;S. Bingrong Zhang;L. Yin;Y. Tian;Y. Ma;A. Zhang;P. Okunieff;L. Zhang
X射线照射小鼠血细胞中mtDNA拷贝数及其转录发生急剧变化，血清中出现mtDNA片段。
DOI：
--
发表时间：
2007
期刊：
Radiatsionnaia biologiia, radioecologiia
影响因子：
--
作者：
E. V. Evdokimovskii;M. Patrushev;T. Ushakova;A. Gaziev
通讯作者：
A. Gaziev
影响因子：
--
作者：
Zhang, Hengshan;Maguire, David;Swarts, Steven;Sun, Weimin;Yang, Shanmin;Wang, Wei;Liu, Chaomei;Zhang, Mei;Zhang, Di;Zhang, Louie;Zhang, Kunzhong;Keng, Peter;Zhang, Lurong;Okunieff, Paul
通讯作者：
Okunieff, Paul