Biofilm Spreading by the Adhesin-Dependent Gliding Motility of Flavobacterium johnsoniae: 2. Role of Filamentous Extracellular Network and Cell-to-Cell Connections at the Biofilm Surface.
Biofilm Spreading by the Adhesin-Dependent Gliding Motility of Flavobacterium johnsoniae: 2. Role of Filamentous Extracellular Network and Cell-to-Cell Connections at the Biofilm Surface.
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DOI：
10.3390/ijms22136911
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发表时间：
2021-06-27
影响因子：
5.6
通讯作者：
Sato C
中科院分区：
文献类型：
--
作者：
Sato K;Naya M;Hatano Y;Kasahata N;Kondo Y;Sato M;Takebe K;Naito M;Sato C
约翰逊黄杆菌在营养贫乏的琼脂上利用滑行运动形成一个薄的扩展菌落。如第一篇论文中所报道的，殖民地中的WT细胞稀疏地包埋在自产的细胞外聚合物基质（ECM）中，而sprB细胞密集地包装在具有较少基质的未成熟生物膜中。殖民地表面对于抗生素抗性和细胞存活至关重要。我们现在已经开发了网格邮票剥离方法，其中殖民地表面附着到TEM网格负染色显微镜。图像显示扩展的凸形WT集落的顶部被具有少量散布细胞的细胞覆盖。暴露于集落殖民地边缘附近的细胞形成头对尾和/或侧对侧接触，有时通过细丝连接。非扩散sprB和gldG和gldK菌落有一个更均匀的上表面覆盖着不同的EPMs，包括囊泡和细丝。sprB、gldG和WT集落的菌落包含直径约2 nm和约5 nm的细丝; gldK集落不包括后者。WT集落边缘附近的每个细胞都有一个或两个黑点，而WT集落内的细胞和SprB、GldG或GldK缺陷集落中的细胞则没有。总之，我们的研究结果表明，殖民地表面结构取决于扩大生物膜的能力。
Flavobacterium johnsoniae forms a thin spreading colony on nutrient-poor agar using gliding motility. As reported in the first paper, WT cells in the colony were sparsely embedded in self-produced extracellular polymeric matrix (EPM), while sprB cells were densely packed in immature biofilm with less matrix. The colony surface is critical for antibiotic resistance and cell survival. We have now developed the Grid Stamp-Peel method whereby the colony surface is attached to a TEM grid for negative-staining microscopy. The images showed that the top of the spreading convex WT colonies was covered by EPM with few interspersed cells. Cells exposed near the colony edge made head-to-tail and/or side-to-side contact and sometimes connected via thin filaments. Nonspreading sprB and gldG and gldK colonies had a more uniform upper surface covered by different EPMs including vesicles and filaments. The EPM of sprB, gldG, and WT colonies contained filaments ~2 nm and ~5 nm in diameter; gldK colonies did not include the latter. Every cell near the edge of WT colonies had one or two dark spots, while cells inside WT colonies and cells in SprB-, GldG-, or GldK-deficient colonies did not. Together, our results suggest that the colony surface structure depends on the capability to expand biofilm.
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影响因子：
10.7
作者：
Loch TP;Faisal M
通讯作者：
Faisal M
影响因子：
4.2
作者：
Laanto E;Penttinen RK;Bamford JK;Sundberg LR
通讯作者：
Sundberg LR
影响因子：
3.2
作者：
Kulkarni, Surashree S.;Johnston, Joseph J.;McBride, Mark J.
通讯作者：
McBride, Mark J.
影响因子：
1.2
作者：
McBride, MJ
通讯作者：
McBride, MJ
影响因子：
4.4
作者：
Gavriilidou, Asimenia;Gutleben, Johanna;Sipkema, Detmer
通讯作者：
Sipkema, Detmer