Antigen presentation by resting B cells. Radiosensitivity of the antigen-presentation function and two distinct pathways of T cell activation.
Antigen presentation by resting B cells. Radiosensitivity of the antigen-presentation function and two distinct pathways of T cell activation.
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DOI：
10.1084/jem.159.3.881
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发表时间：
1984-03-01
期刊：
影响因子：
--
通讯作者：
Schwartz RH
中科院分区：
文献类型：
--
作者：
Ashwell JD;DeFranco AL;Paul WE;Schwartz RH
在本报告中，我们通过T细胞增殖或T细胞依赖的B细胞刺激，研究了静止的小B细胞作为抗原呈递细胞（APC）对抗原特异性MHC限制性T细胞的能力。我们发现，14个体外抗原特异性mhc限制性T细胞克隆和系中的10个，以及4个T细胞杂交瘤中的3个，在轻度辐照（1000拉德）纯化的B细胞和适当的外源抗原存在下，可以诱导增殖或分泌IL-2。所有的T细胞系和杂交瘤都被巨噬细胞和树突状细胞富集的群体刺激增殖或产生IL-2，除了一个杂交瘤外，所有的T细胞在被B细胞作为APC刺激时都引起B细胞活化。此外，轻度照射、高度纯化的同源B细胞与低密度树突状和巨噬细胞一样，都是APC诱导B细胞活化的有效来源。当与纯化的B细胞和适当的抗原一起培养时，也发现从抗原引物动物身上新鲜取出的淋巴结T细胞增殖。因此，小的静止B细胞可以作为APC对多种T细胞起作用。APC功能在1000拉德照射下很容易检测到，但在3300拉德照射时，APC功能明显减弱或缺失。因此，其他一些实验室未能观察到这一现象可能是由于B细胞抗原呈递功能的相对放射敏感性。此外，这种放射敏感性使我们能够很容易地区分B细胞抗原呈递与树突状细胞和巨噬细胞呈递，因为后者耐3300拉德。最后，当B细胞被用作APC时，一个不能增殖的T细胞克隆能够在同基因B细胞和适当抗原存在的情况下招募异体B细胞增殖。这一结果表明，在T细胞中至少有两种不同的激活途径，一种导致T细胞增殖，另一种导致B细胞募集因子的分泌。
In this report we have examined the ability of small resting B cells to act as antigen-presenting cells (APC) to antigen-specific MHC- restricted T cells as assessed by either T cell proliferation or T cell- dependent B cell stimulation. We found that 10 of 14 in vitro antigen- specific MHC-restricted T cell clones and lines and three of four T cell hybridomas could be induced to either proliferate or secrete IL-2 in the presence of lightly irradiated (1,000 rads) purified B cells and the appropriate foreign antigen. All T cell lines and hybridomas were stimulated to proliferate or make IL-2 by macrophage- and dendritic cell-enriched populations and all T cells tested except one hybridoma caused B cell activation when stimulated with B cells as APC. Furthermore, lightly irradiated, highly purified syngeneic B cells were as potent a source of APC for inducing B cell activation as were low density dendritic and macrophage-enriched cells. Lymph node T cells freshly taken from antigen-primed animals were also found to proliferate when cultured with purified B cells and the appropriate antigen. Thus, small resting B cells can function as APC to a variety of T cells. This APC function was easily measured when the cells were irradiated with 1,000 rads, but was greatly diminished or absent when they were irradiated with 3,300 rads. Thus, the failure of some other laboratories to observe this phenomenon may be the result of the relative radiosensitivity of the antigen-presenting function of the B cells. In addition, this radiosensitivity allowed us to easily distinguish B cell antigen presentation from presentation by the dendritic cell and macrophage, as the latter was resistant to 3,300 rads. Finally, one T cell clone that failed to proliferate when B cells were used as APC was able to recruit allogeneic B cells to proliferate in the presence of syngeneic B cells and the appropriate antigen. This result suggests that there are at least two distinct pathways of activation in T cells, one that leads to T cell proliferation and one that leads to the secretion of B cell recruitment factor(s).
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影响因子：
15.3
作者：
BANDEIRA, A;POBOR, G;COUTINHO, A
通讯作者：
COUTINHO, A
影响因子：
15.3
作者：
KIMOTO, M;FATHMAN, CG
通讯作者：
FATHMAN, CG
影响因子：
5.4
作者：
ANDERSON, RE;SPRENT, J;MILLER, JFAP
通讯作者：
MILLER, JFAP
影响因子：
15.3
作者：
MCKEAN, DJ;INFANTE, AJ;WARNER, N
通讯作者：
WARNER, N
DOI：
10.1073/pnas.80.19.6000
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
COWING, C;CHAPDELAINE, JM
通讯作者：
CHAPDELAINE, JM