Human umbilical endothelial cells (HUVECs) have a sex: characterisation of the phenotype of male and female cells.
Human umbilical endothelial cells (HUVECs) have a sex: characterisation of the phenotype of male and female cells.
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DOI：
10.1186/s13293-014-0018-2
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发表时间：
2014
影响因子：
7.9
通讯作者：
Franconi F
中科院分区：
文献类型：
--
作者：
Addis R;Campesi I;Fois M;Capobianco G;Dessole S;Fenu G;Montella A;Cattaneo MG;Vicentini LM;Franconi F
人脐静脉内皮细胞（HUVECs）被广泛用于研究可能涉及心血管水平上检测到的性别和性别差异的内皮生理和病理。本研究评估了HUVECs在形态、增殖和迁移特性以及雌激素和雄激素受体和一氧化氮合酶3（NOS 3）的基因和蛋白表达方面是否具有性二态性。此外，由于自噬受性别影响，因此在雄性和雌性HUVEC（MHUVEC和FHUVEC）中分析其程度。研究了健康、正常体重的男性和女性新生儿的脐带，这些新生儿的母亲是健康、不肥胖和不吸烟的女性。HUVEC形态学通过电子显微镜分析，其功能通过增殖、活力、伤口愈合和趋化性测定进行研究。分别通过实时PCR和Western blotting评估雌激素和雄激素受体以及NOS 3的基因和蛋白表达，并通过Western blotting检测参与自噬调节的主要分子[蛋白激酶B（Akt）、哺乳动物雷帕霉素靶蛋白（mTOR）、beclin-1和微管相关蛋白1轻链3（LC 3）]的表达。FHUVECs的细胞增殖、迁移、NOS 3 mRNA和蛋白表达均显著高于MHUVECs。相反，beclin-1和LC 3-II/LC 3-I比率在MHUVEC中高于FHUVEC，表明雄性细胞比雌性细胞更具自噬性。雌激素和雄激素受体基因和蛋白的表达、Akt和mTOR蛋白的表达以及细胞大小和形状不受性别影响。男女新生儿体重差异不显著，但男婴体重与母亲体重呈正相关，提示母亲体重对男婴和女婴的影响可能不同。结果表明，性别差异存在于产前生活和参数特异性，这表明，HUVECs的两种性别应被用作体外模型，以提高质量和研究的转化价值。在HUVEC中观察到的性别差异可能与解释成年期疾病有关，因为内皮功能障碍在心血管疾病、糖尿病、神经退行性疾病和免疫疾病的发病机制中起着至关重要的作用。
Human umbilical endothelial cells (HUVECs) are widely used to study the endothelial physiology and pathology that might be involved in sex and gender differences detected at the cardiovascular level. This study evaluated whether HUVECs are sexually dimorphic in their morphological, proliferative and migratory properties and in the gene and protein expression of oestrogen and androgen receptors and nitric oxide synthase 3 (NOS3). Moreover, because autophagy is influenced by sex, its degree was analysed in male and female HUVECs (MHUVECs and FHUVECs). Umbilical cords from healthy, normal weight male and female neonates born to healthy non-obese and non-smoking women were studied. HUVEC morphology was analysed by electron microscopy, and their function was investigated by proliferation, viability, wound healing and chemotaxis assays. Gene and protein expression for oestrogen and androgen receptors and for NOS3 were evaluated by real-time PCR and Western blotting, respectively, and the expression of the primary molecules involved in autophagy regulation [protein kinase B (Akt), mammalian target of rapamycin (mTOR), beclin-1 and microtubule-associated protein 1 light chain 3 (LC3)] were detected by Western blotting. Cell proliferation, migration NOS3 mRNA and protein expression were significantly higher in FHUVECs than in MHUVECs. Conversely, beclin-1 and the LC3-II/LC3-I ratio were higher in MHUVECs than in FHUVECs, indicating that male cells are more autophagic than female cells. The expression of oestrogen and androgen receptor genes and proteins, the protein expression of Akt and mTOR and cellular size and shape were not influenced by sex. Body weights of male and female neonates were not significantly different, but the weight of male babies positively correlated with the weight of the mother, suggesting that the mother’s weight may exert a different influence on male and female babies. The results indicate that sex differences exist in prenatal life and are parameter-specific, suggesting that HUVECs of both sexes should be used as an in vitro model to increase the quality and the translational value of research. The sex differences observed in HUVECs could be relevant in explaining the diseases of adulthood because endothelial dysfunction has a crucial role in the pathogenesis of cardiovascular diseases, diabetes mellitus, neurodegeneration and immune disease.
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影响因子：
2.1
作者：
Lyros, Epameinondas;Bakogiannis, Constantinos;Fassbender, Klaus
通讯作者：
Fassbender, Klaus
影响因子：
3.7
作者：
de Jonge HJ;Fehrmann RS;de Bont ES;Hofstra RM;Gerbens F;Kamps WA;de Vries EG;van der Zee AG;te Meerman GJ;ter Elst A
通讯作者：
ter Elst A
DOI：
10.1042/bj20111451
发表时间：
2012-01-15
期刊：
The Biochemical journal
影响因子：
--
作者：
Lee J;Giordano S;Zhang J
通讯作者：
Zhang J
影响因子：
3.7
作者：
Cattaneo, Maria Grazia;Cappellini, Elisa;Vicentini, Lucia M.
通讯作者：
Vicentini, Lucia M.
影响因子：
4.8
作者：
Evans, MJ;Harris, HA;Adelman, SJ
通讯作者：
Adelman, SJ