Wound-induced pectin methylesterases enhance banana (Musa spp. AAA) susceptibility to Fusarium oxysporum f. sp. cubense.
Wound-induced pectin methylesterases enhance banana (Musa spp. AAA) susceptibility to Fusarium oxysporum f. sp. cubense.
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DOI：
10.1093/jxb/ert088
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发表时间：
2013-05
影响因子：
6.9
通讯作者：
Xu C
中科院分区：
文献类型：
--
作者：
Ma L;Jiang S;Lin G;Cai J;Ye X;Chen H;Li M;Li H;Takác T;Samaj J;Xu C
最近的研究表明，植物果胶甲基酯酶（PME）除了在植物发育中发挥作用外，还直接参与植物防御。然而，PME对果胶作用的分子机制还不清楚。为了了解PMEs如何改变香蕉（Musa spp.）- 镰刀菌互作，比较了高抗和感病香蕉品种PMEs的表达和酶活性。此外，还研究了PME的空间分布及其对10个具有不同甲基化程度（DM）的单独的同型半乳糖醛酸（HG）表位的果胶甲基化的影响。结果表明，在病原菌处理前，抗病品种表现出比感病品种高的果胶酶活性，与低水平的果胶DM相对应。PME的表达和活性的显着增加和果胶DM的减少，观察在感病品种，但不是在抗性品种时，植物受伤，这是必要的成功感染。随着感病品种PME的增加，JIM 5抗原（低甲基化HGs）也随之增加。病原菌感染后48小时，感病品种的PME活性和表达量高于抗病品种，而DM则低于抗病品种。总之，抗、感品种对伤害的反应存在显著差异。增加PMEs，然后减少DMs伴随着增加低甲基化的HGs在根维管柱似乎起着关键作用，在确定香蕉对镰刀菌的敏感性。
Recent studies suggest that plant pectin methylesterases (PMEs) are directly involved in plant defence besides their roles in plant development. However, the molecular mechanisms of PME action on pectins are not well understood. In order to understand how PMEs modify pectins during banana (Musa spp.)–Fusarium interaction, the expression and enzyme activities of PMEs in two banana cultivars, highly resistant or susceptible to Fusarium, were compared with each other. Furthermore, the spatial distribution of PMEs and their effect on pectin methylesterification of 10 individual homogalacturonan (HG) epitopes with different degrees of methylesterification (DMs) were also examined. The results showed that, before pathogen treatment, the resistant cultivar displayed higher PME activity than the susceptible cultivar, corresponding well to the lower level of pectin DM. A significant increase in PME expression and activity and a decrease in pectin DM were observed in the susceptible cultivar but not in the resistant cultivar when plants were wounded, which was necessary for successful infection. With the increase of PME in the wounded susceptible cultivar, the JIM5 antigen (low methyestrified HGs) increased. Forty-eight hours after pathogen infection, the PME activity and expression in the susceptible cultivar were higher than those in the resistant cultivar, while the DM was lower. In conclusion, the resistant and the susceptible cultivars differ significantly in their response to wounding. Increased PMEs and thereafter decreased DMs acompanied by increased low methylesterified HGs in the root vascular cylinder appear to play a key role in determination of banana susceptibility to Fusarium.
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影响因子：
7.4
作者：
LINERS, F;LETESSON, JJ;VANCUTSEM, P
通讯作者：
VANCUTSEM, P
影响因子：
1.2
作者：
Salerno, Maria-Isabel;Gianinazzi, Silvio;Gianinazzi-Pearson, Vivienne
通讯作者：
Gianinazzi-Pearson, Vivienne
影响因子：
7.2
作者：
Osorio, Sonia;Castillejo, Cristina;Valpuesta, Victoriano
通讯作者：
Valpuesta, Victoriano
影响因子：
4.3
作者：
Boudjeko, T;Andème-Onzighi, C;Driouich, A
通讯作者：
Driouich, A
影响因子：
3.8
作者：
Louvet, Romain;Rayon, Catherine;Pelloux, Jerome
通讯作者：
Pelloux, Jerome