Preparation and characterization of [N alpha-(4-azido-2-nitrophenyl)Ala1,Tyr36]-parathyroid hormone related peptide (1-36)amide: a high-affinity, partial agonist having high cross-linking efficiency with its receptor on ROS 17/2.8 cells.
Preparation and characterization of [N alpha-(4-azido-2-nitrophenyl)Ala1,Tyr36]-parathyroid hormone related peptide (1-36)amide: a high-affinity, partial agonist having high cross-linking efficiency with its receptor on ROS 17/2.8 cells.
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[N α-(4-叠氮基-2-硝基苯基)Ala1,Tyr36]-甲状旁腺激素相关肽(1-36)酰胺的制备和表征：一种与其受体具有高交联效率的高亲和力部分激动剂
DOI：
10.1021/bi00482a001
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Segre,GV
中科院分区：
文献类型：
--
作者：
Jüppner,H;Abou-Samra,AB;Uneno,S;Keutmann,HT;PottsJr,JT;Segre,GV
材料与方法一种含有酪氨酸的PTHrP类似物，[Tyr36] PTHrP （l-36）酰胺，由康涅狄格州纽黑文耶鲁大学医学院的A. F. Stewart -art博士提供（Stewart等人，1988）。[Nle8'18, Tyr34] bPTH (l-34) amide来自Bachem Fine Chemicals （Torrance， CA）。3-异丁基-1-甲基黄嘌呤（IMBX）来自Sigma （St. Louis， MO）。FNPA、三乙胺（TEA）、三氟乙酸（TFA）和七氟丁酸（HFBA）来自Pierce (Rockwood, 1L)，二甲亚砜（DMSO）来自Aldrich （Milwaukee， WI）。内源性蛋白酶gluc购自Boehringer， Mannheim （Mannheim, West Germany）。SDS-PAGE预混合低分子量标准品来自Pharmacia （Uppsala, Sweden）。琥珀酰化cAMP抗血清由Dr. Kevin Catt， NIH, Bethesda， MD提供。钠[125I]碘化钠（2200 Ci/mmol）由e.i. Du Pont de Nemours and Co., Inc.（Boston， MA）提供。510型高效液相色谱泵，680型自动梯度前，440型吸光度检测器，和/uBondapak C-18柱（3.9毫米X 30厘米）来自Waters （Bedford， MA）。本研究中使用的所有其他试剂均为可用的最高等级试剂。蛋白质测序使用Applied Biosystems （Foster City， CA）的溶剂和试剂，使用Applied Biosystems Model 477A蛋白/肽测序仪。应用应用生物系统120A PTH模型对苯硫代海因（PTH）氨基酸衍生物进行了在线分析
Materials and Methods Materials. A tyrosine-containing analogue of PTHrP,[Tyr36] PTHrP (l-36) amide, was provided by Dr. A. F. Stew-art, Yale University School of Medicine, New Haven, CT (Stewart et al., 1988).[Nle8'18, Tyr34] bPTH (l-34) amide was from Bachem Fine Chemicals (Torrance, CA). 3-Isobutyl-1-methylxanthine (IMBX) was from Sigma (St. Louis, MO). FNPA, triethylamine (TEA), trifluoroacetic acid (TFA), and heptafluorobutyric acid (HFBA) were from Pierce (Rockwood, 1L), and dimethyl sulfoxide (DMSO) was from Aldrich (Milwaukee, WI). Endoproteinase Glu-C was purchased from Boehringer, Mannheim (Mannheim, West Germany). Pre-mixed low molecular weight standards for SDS-PAGE were from Pharmacia (Uppsala, Sweden). The antiserum against succinylated cAMP was provided by Dr. Kevin Catt, NIH, Bethesda, MD. Sodium [125I] iodide (2200 Ci/mmol) was obtained from E. I. Du Pont de Nemours and Co., Inc.(Boston, MA). Model 510 HPLC pumps, model 680 auto-matic gradient former, model 440 absorbance detector, and/uBondapak C-18 columns (3.9 mm X 30 cm) were from Waters (Bedford, MA). All other reagents employed in this study were of the highest grade available. Protein sequencing was performed with solvents and reag-ents from Applied Biosystems (Foster City, CA) on an Applied Biosystems Model 477A protein/peptide sequencer. The phenylthiohydantoin (PTH) amino acid derivatives were an-alyzed on-line with an Applied Biosystems Model 120A PTH
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甲状旁腺激素光反应类似物的制备 [Nle8, Lys(N-ε-4-azido-2-硝基苯基)13,Nle18,Tyr34]牛甲状旁腺激素-(1-34)NH2，一种选择性、高亲和力配体
影响因子：
2.9
作者：
C. Shigeno;Y. Hiraki;H. Keutmann;A. M. Stern;J. Potts;G. Segre
通讯作者：
G. Segre
克隆大鼠骨肉瘤细胞中甲状旁腺激素受体的表征和激动剂诱导的下调。
影响因子：
4.8
作者：
I. Yamamoto;C. Shigeno;J. Potts;G. Segre
通讯作者：
G. Segre
糖皮质激素增加大鼠成骨细胞骨肉瘤细胞中的甲状旁腺激素受体 (ROS 17/2)。
DOI：
10.1002/jbmr.5650030617
发表时间：
1988
期刊：
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
影响因子：
--
作者：
Yamamoto,I;PottsJr,JT;Segre,GV
通讯作者：
Segre,GV
人类肿瘤合成肽与甲状旁腺激素在体内和体外的相似性。
DOI：
10.1126/science.3685994
发表时间：
1987
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Horiuchi,N;Caulfield,MP;Fisher,JE;Goldman,ME;McKee,RL;Reagan,JE;Levy,JJ;Nutt,RF;Rodan,SB;Schofield,TL
通讯作者：
Schofield,TL
克隆大鼠骨肉瘤细胞中甲状旁腺激素受体的光亲和标记。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Shigeno,C;Hiraki,Y;Westerberg,DP;PottsJr,JT;Segre,GV
通讯作者：
Segre,GV