Spatially non-uniform condensates emerge from dynamically arrested phase separation.
Spatially non-uniform condensates emerge from dynamically arrested phase separation.
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DOI：
10.1038/s41467-023-36059-1
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发表时间：
2023-02-08
影响因子：
16.6
通讯作者：
Knowles, Tuomas P. J.
中科院分区：
文献类型：
--
作者：
Erkamp, Nadia A.;Sneideris, Tomas;Ausserwoger, Hannes;Qian, Daoyuan;Qamar, Seema;Nixon-Abell, Jonathon;St George-Hyslop, Peter;Schmit, Jeremy D.;Weitz, David A.;Knowles, Tuomas P. J.
通过蛋白质和核酸的相分离形成生物分子凝聚体正成为活细胞广泛使用的空间组织原理。然而，许多这样的生物分子凝聚体不是均匀的流体，而是具有由不同成分的不同子隔间组成的内部结构。值得注意的是，凝析油可以包含在组成凝析油的生物聚合物中耗尽的隔室。在这里，我们证明了这种双乳凝析物是通过动态捕获的相变产生的。组合物的变化加上对这种变化的缓慢反应的结合可能导致富聚合物凝析相中缺乏生物聚合物的液滴成核。我们的研究结果表明，具有复杂内部结构的凝析物可能来自动力学驱动力，而不是纯粹的热力学驱动力，并为了解和控制体外和体内凝析物的内部结构提供了更广泛的途径。具有内部结构的生物分子凝聚物允许细胞进一步组织其过程。在这项工作中，作者研究了冷凝物如何通过动力学而不是纯粹的热力学驱动力获得内部有稀相液滴的内部结构。
The formation of biomolecular condensates through phase separation from proteins and nucleic acids is emerging as a spatial organisational principle used broadly by living cells. Many such biomolecular condensates are not, however, homogeneous fluids, but possess an internal structure consisting of distinct sub-compartments with different compositions. Notably, condensates can contain compartments that are depleted in the biopolymers that make up the condensate. Here, we show that such double-emulsion condensates emerge via dynamically arrested phase transitions. The combination of a change in composition coupled with a slow response to this change can lead to the nucleation of biopolymer-poor droplets within the polymer-rich condensate phase. Our findings demonstrate that condensates with a complex internal architecture can arise from kinetic, rather than purely thermodynamic driving forces, and provide more generally an avenue to understand and control the internal structure of condensates in vitro and in vivo. Biomolecular condensates with internal structure allow cells to further organise their processes. In this work the authors investigate how condensates can obtain an internal structure with droplets of dilute phase inside via kinetic, rather than purely thermodynamic driving forces.
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影响因子：
64.5
作者：
Feric M;Vaidya N;Harmon TS;Mitrea DM;Zhu L;Richardson TM;Kriwacki RW;Pappu RV;Brangwynne CP
通讯作者：
Brangwynne CP
DOI：
10.1083/jcb.202009079
发表时间：
2021-03-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Freibaum BD;Messing J;Yang P;Kim HJ;Taylor JP
通讯作者：
Taylor JP
DOI：
10.1073/pnas.1504822112
发表时间：
2015-06-09
影响因子：
11.1
作者：
Elbaum-Garfinkle, Shana;Kim, Younghoon;Brangwynne, Clifford P.
通讯作者：
Brangwynne, Clifford P.
影响因子：
--
作者：
MAHOWALD, AP
通讯作者：
MAHOWALD, AP
影响因子：
3.9
作者：
Chen, Nannan;Zhang, Jinglin;Wang, Qin
通讯作者：
Wang, Qin