Features governing symbiont persistence in the squid-vibrio association.
Features governing symbiont persistence in the squid-vibrio association.
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DOI：
10.1111/mec.12474
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发表时间：
2014-03
影响因子：
4.9
通讯作者：
Ruby EG
中科院分区：
文献类型：
--
作者：
Koch EJ;Miyashiro T;McFall-Ngai MJ;Ruby EG
在成熟的共生器官中，宿主与共生体相互作用的实验研究对大多数动物-细菌关系提出了挑战。寄主乌贼Euprymna scoloes的饲养进展使我们能够通过实验定植特定菌株的共生费氏弧菌（Vibrio fischeri）来探索共生体发光缺陷与后期发育（例如共生体持久性和组织形态发生）之间的关系。幼乌贼接种后4周内，野生型菲氏弧菌的数量增加了100倍；相比之下，一个产光缺陷菌株（Δlux）在第一天正常定植，但在随后的几周内表现出指数下降到无法检测的水平。两种菌株的共定殖既不影响野生型的定殖轨迹，也不影响Δlux水平的下降。未受感染的动物在孵化后至少两周内保留了被定植的能力。然而，一旦被野生型菌株定殖5天，随后的实验诱导的共生体损失不能成功地再定殖，这表明宿主进入了一个难治状态。然而，在失去共生体之前被Δlux殖民的动物是容易接受重新殖民的。对野生型或Δlux菌株定殖的动物的分析显示，促进定殖过程的纤毛轻器官组织的发育退化略有差异。因此，Δlux菌株缺陷的一些其他特征也可能是其无法持续存在的原因，也可能是其无法在宿主体内诱导难解状态的原因。
Experimental studies of the interaction between host and symbiont in a maturing symbiotic organ have presented a challenge for most animal-bacterial associations. Advances in the rearing of the host squid Euprymna scolopes have enabled us to explore the relationship between a defect in symbiont light production and late-stage development (e.g., symbiont persistence and tissue morphogenesis) by experimental colonization with specific strains of the symbiont Vibrio fischeri. During the first four weeks post-inoculation of juvenile squid, the population of wild-type V. fischeri increased 100-fold; in contrast, a strain defective in light production (Δlux) colonized normally the first day, but exhibited an exponential decline to undetectable levels over subsequent weeks. Co-colonization of organs by both strains affected neither the trajectory of colonization by wild type, nor the decline of Δlux levels. Uninfected animals retained the ability to be colonized for at least two weeks post-hatch. However, once colonized by the wild-type strain for 5 days, a subsequent experimentally induced loss of the symbionts could not be followed by a successful recolonization, indicating the host’s entry into a refractory state. However, animals colonized by the Δlux before the loss of their symbionts were receptive to recolonization. Analyses of animals colonized with either a wild-type or a Δlux strain revealed slight, if any, differences in the developmental regression of the ciliated light-organ tissues that facilitate the colonization process. Thus, some other feature(s) of the Δlux strain’s defect also may be responsible for its inability to persist, and its failure to induce a refractory state in the host.
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影响因子：
3.4
作者：
Chaston JM;Murfin KE;Heath-Heckman EA;Goodrich-Blair H
通讯作者：
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影响因子：
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作者：
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通讯作者：
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作者：
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