Adherence of Escherichia coli O157:H7 Mutants In Vitro and in Ligated Pig Intestines
Adherence of Escherichia coli O157:H7 Mutants In Vitro and in Ligated Pig Intestines
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大肠杆菌 O157:H7 突变体在体外和结扎猪肠中的粘附
DOI：
10.1128/aem.00297-09
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发表时间：
2009-06
影响因子：
4.4
通讯作者：
Zhu, Jing
中科院分区：
文献类型：
--
作者：
Chambers, James R.;Liu, Bianfang（刘变芳）;Johnson, Roger P.;Yin, Xianhua;Wheatcroft, Roger;Gyles, Carlton L.;Zhu, Jing
关于Verotoxin（VT）在肠出血性大肠杆菌O 157：H7（O 157 EHEC）粘附于肠上皮细胞中的作用，文献报道相互矛盾。据报道，该病原体的O岛7（OI-7）、OI-15和OI-48的推定毒力基因也可能影响体外粘附。因此，产生了vt 2和OI-7的片段以及基因aidA 15（来自OI-15的基因）和aidA 48（来自OI-48的基因）的突变体，并评价了体外对培养的人HEp-2和猪空肠上皮（IPEC-J2）细胞的粘附性以及体内对猪回肠袢中肠上皮细胞的粘附性。VT-2阴性突变体的粘附HEp-2和IPEC-J2细胞和肠上皮细胞在猪回肠环显着下降;互补只部分恢复VT-2生产，但完全恢复了对野生型水平的粘附培养细胞。OI-7和aidA 48的缺失对粘附没有影响，而aidA 15的缺失导致猪回肠袢中的粘附显著降低，但对培养的细胞没有影响。本研究支持VT 2在粘附中起作用的发现，表明E. coli O 157：H7的粘附能力可能与体外获得的粘附能力不同，并确定AIDA-15在E.大肠杆菌O 157：H7。
ABSTRACT There are contradictory literature reports on the role of verotoxin (VT) in adherence of enterohemorrhagic Escherichia coli O157:H7 (O157 EHEC) to intestinal epithelium. There are reports that putative virulence genes of O island 7 (OI-7), OI-15, and OI-48 of this pathogen may also affect adherence in vitro. Therefore, mutants of vt2 and segments of OI-7 and genes aidA15 (gene from OI-15) and aidA48 (gene from OI-48) were generated and evaluated for adherence in vitro to cultured human HEp-2 and porcine jejunal epithelial (IPEC-J2) cells and in vivo to enterocytes in pig ileal loops. VT2-negative mutants showed significant decreases in adherence to both HEp-2 and IPEC-J2 cells and to enterocytes in pig ileal loops; complementation only partially restored VT2 production but fully restored the adherence to the wild-type level on cultured cells. Deletion of OI-7 and aidA48 had no effect on adherence, whereas deletion of aidA15 resulted in a significant decrease in adherence in pig ileal loops but not to the cultured cells. This investigation supports the findings that VT2 plays a role in adherence, shows that results obtained in adherence of E. coli O157:H7 in vivo may differ from those obtained in vitro, and identified AIDA-15 as having a role in adherence of E. coli O157:H7.
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影响因子：
--
作者：
Murphy KC;Campellone KG
通讯作者：
Campellone KG
影响因子：
5.1
作者：
T. Wells;O. Sherlock;Lucy Rivas;A. Mahajan;S. Beatson;M. Torpdahl;R. Webb;L. Allsopp;K. Gobius;D. Gally;M. Schembri
通讯作者：
T. Wells;O. Sherlock;Lucy Rivas;A. Mahajan;S. Beatson;M. Torpdahl;R. Webb;L. Allsopp;K. Gobius;D. Gally;M. Schembri
影响因子：
3.2
作者：
R. Ménard;P. Sansonetti;C. Parsot
通讯作者：
R. Ménard;P. Sansonetti;C. Parsot
DOI：
10.1016/b978-0-12-384939-7.10005-5
发表时间：
2012-02-29
期刊：
Principles of Molecular Virology
影响因子：
--
作者：
Cann AJ
通讯作者：
Cann AJ
影响因子：
3.1
作者：
Gunzer, F;Bohn, U;Donohue-Rolfe, A
通讯作者：
Donohue-Rolfe, A