Qing-Re-Xiao-Zheng Formula Modulates Gut Microbiota and Inhibits Inflammation in Mice With Diabetic Kidney Disease.
Qing-Re-Xiao-Zheng Formula Modulates Gut Microbiota and Inhibits Inflammation in Mice With Diabetic Kidney Disease.
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清热消症方可调节糖尿病肾病小鼠的肠道微生物群并抑制炎症。
DOI：
10.3389/fmed.2021.719950
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发表时间：
2021
影响因子：
3.9
通讯作者：
Peng L
中科院分区：
文献类型：
--
作者：
Gao Y;Yang R;Guo L;Wang Y;Liu WJ;Ai S;Woon TH;Wang Z;Zhai Y;Wang Z;Peng L
有证据表明，肠道微生物群失调引起的代谢炎症会导致糖尿病肾病。补充益生元可预防 DKD 患者肠道微生物群失调、抑制炎症反应并保护肾功能。清热消症方（QRXZF）是一种中药方剂，在中国已用于治疗糖尿病肾病。最近，越来越多的研究表明，调节肠道微生物群是 DKD 的潜在治疗策略，因为它能够减少与 DKD 相关的代谢炎症。然而，QRXZF 是否通过调节肠道微生物群对 DKD 有效尚不清楚。在本研究中，我们通过探索 QRXZF 肠道微生物群与肾脏中肠源性脂多糖 (LPS) 介导的下游炎症通路之间的潜在机制，研究了 QRXZF 的肾脏保护作用。建立高脂饮食（HFD）和链脲佐菌素注射诱导的 DKD 小鼠模型以评估 QRXZF 的体内作用。用 QRXZF 治疗 8 周的小鼠的尿白蛋白、血清胆固醇和甘油三酯水平显着降低。通过组织学分析观察到的肾损伤也减轻了。此外，与 DKD 组相比，QRXZF 组小鼠的闭锁小带蛋白 1 (ZO-1) 表达水平较高，血清异硫氰酸荧光素 (FITC)-葡聚糖水平较低，结肠粘膜损伤较少，这意味着对肠道屏障完整性有好处。 QRXZF 治疗还逆转了肠道菌群失调并降低了肠道来源的 LPS 水平。值得注意的是，Toll 样受体 4 (TLR4) 和核因子-κB (NF-κB) 的表达在 QRXZF 组中受到抑制，这两种信号是 DKD 中重要的炎症途径。总之，我们的结果表明 QRXZF 的肾脏保护作用可能与调节肠道微生物群和抑制肾脏炎症反应有关。
Evidence indicates that the metabolic inflammation induced by gut microbiota dysbiosis contributes to diabetic kidney disease. Prebiotic supplementations to prevent gut microbiota dysbiosis, inhibit inflammatory responses, and protect the renal function in DKD. Qing-Re-Xiao-Zheng formula (QRXZF) is a Traditional Chinese Medicine (TCM) formula that has been used for DKD treatment in China. Recently, there are growing studies show that regulation of gut microbiota is a potential therapeutic strategy for DKD as it is able to reduce metabolic inflammation associated with DKD. However, it is unknown whether QRXZF is effective for DKD by regulating of gut microbiota. In this study, we investigated the reno-protective effect of QRXZF by exploring its potential mechanism between gut microbiota and downstream inflammatory pathways mediated by gut-derived lipopolysaccharide (LPS) in the kidney. High-fat diet (HFD) and streptozotocin injection-induced DKD mice model was established to assess the QRXZF effect in vivo. Mice treated with QRXZF for 8 weeks had significantly lower levels of urinary albumin, serum cholesterol and triglycerides. The renal injuries observed through histological analysis were attenuated as well. Also, mice in the QRXZF group had higher levels of Zonula occludens protein-1 (ZO-1) expression, lower levels of serum fluorescein-isothiocyanate (FITC)-dextran and less-damaged colonic mucosa as compared to the DKD group, implying the benefit role for the gut barrier integrity. QRXZF treatment also reversed gut dysbiosis and reduced levels of gut-derived LPS. Notably, the expression of toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB), which are important inflammation pathways in DKD, were suppressed in the QRXZF groups. In conclusion, our results indicated that the reno-protective effects of QRXZF was probably associated with modulating gut microbiota and inhibiting inflammatory responses in the kidney.
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影响因子：
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作者：
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DOI：
10.2147/dddt.s150825
发表时间：
2017
期刊：
Drug design, development and therapy
影响因子：
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作者：
Li L;Ma L;Fu P
通讯作者：
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