Regulation of multiple basic fibroblast growth factor messenger ribonucleic acid transcripts by protein kinase C activators.
Regulation of multiple basic fibroblast growth factor messenger ribonucleic acid transcripts by protein kinase C activators.
复制标题
蛋白激酶 C 激活剂对多种碱性成纤维细胞生长因子信使核糖核酸转录物的调节。
作者：
P. Murphy;Y. Sato;R. Sato;H. Friesen
人脑星形细胞瘤细胞株U87-MG表达两个主要的碱性成纤维细胞生长因子基因转录产物，分别为7.0和3.7kb，以及几个低丰度的低分子转录本(1.0-1.8kb)。佛波酯佛波酯-12，13-二丁酸酯引起碱性成纤维细胞生长因子基因转录本的丰度随时间和剂量的增加而增加。浓度为1微米时，佛波酯在4h内使7.0和3.7kb转录本的丰度均增加，6h时达到平台期，是对照水平的1.5~2.5倍，并持续升高至少12h。血小板衍生生长因子(0.15-5U/ml)或合成二酰甘油类似物1-油酰基-2-乙酰-RAC甘油(1-300 nM)也能刺激这些细胞的DNA合成。(Bu)2cAMP(0.03-2 mM)和A23187(0.3-1000 nM)对成纤维细胞生长因子的表达均无影响。当U87-MG细胞暴露于佛波酯24小时时，观察到几个不同之处：剂量反应曲线左移(EC50=3-5 nM；最大反应在10 nM时)，7.0和3.7kb转录本的反应在较高剂量(100-1000 nM)时减弱，可能反映了佛波酯下调蛋白激酶C的表达。
The human astrocytoma cell line U87-MG expressed two major basic fibroblast growth factor (FGF) mRNA transcripts of 7.0 and 3.7 kilobase (kb), as well as several low abundance transcripts of lower mol wt (1.0-1.8 kb). The phorbol ester phorbol-12,13-dibutyrate caused a time- and dose-dependent increase in the abundance of basic FGF mRNA transcripts. At a concentration of 1 microM, phorbol ester increased the level of both the 7.0 and 3.7 kb transcripts within 4 h, reached a plateau at 1.5- to 2.5-fold above control levels by 6 h and remained elevated for at least 12 h. When measured at 6 h after drug addition, the abundance of both 7.0 and 3.7 kb transcripts was maximally stimulated by 100 nM phorbol ester (EC50 = 10-20 nM). FGF mRNA levels were also stimulated to a similar extent by platelet-derived growth factor (0.15-5 U/ml) or the synthetic diacylglycerol analog 1-oleoyl-2-acetyl-rac glycerol (1-300 nM) at doses which stimulated DNA synthesis in these cells. Neither (Bu)2cAMP (0.03-2 mM) nor A23187 (0.3-1000 nM) had any effect on FGF expression. When U87-MG cells were exposed to phorbol ester for 24 h several differences were observed: the dose response curve was shifted to the left (EC50 = 3-5 nM; maximum response at 10 nM phorbol ester) and the response of the 7.0 and 3.7 kb transcripts was attenuated at higher doses (100-1000 nM), perhaps reflecting down-regulation of protein kinase C by the phorbol ester.(ABSTRACT TRUNCATED AT 250 WORDS)
鉴定负责增强乳腺肿瘤细胞中纤溶酶原激活剂活性的垂体因子。
DOI：
10.1073/pnas.83.20.7780
发表时间：
1986
影响因子：
11.1
作者：
Mira-y-Lopez,R;Joseph-Silverstein,J;Rifkin,DB;Ossowski,L
通讯作者：
Ossowski,L
3T3-L1 细胞中生长因子刺激的蛋白质磷酸化。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Blackshear,PJ;Witters,LA;Girard,PR;Kuo,JF;Quamo,SN
通讯作者：
Quamo,SN