The GABAA receptor is expressed in human neurons derived from a teratocarcinoma cell line.
The GABAA receptor is expressed in human neurons derived from a teratocarcinoma cell line.
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GABAA 受体在源自畸胎癌细胞系的人类神经元中表达。
DOI：
10.1006/bbrc.1997.7087
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发表时间：
1997
影响因子：
3.1
通讯作者：
T. Nishizaki
中科院分区：
文献类型：
--
作者：
T. Matsuoka;T. Kondoh;N. Tamaki;T. Nishizaki
NT2 细胞是一种人畸胎癌细胞系，经视黄酸处理后可分化为神经元样细胞（NT2-N 细胞）。本研究使用膜片钳记录鉴定了 NT2-N 细胞中表达的神经递质受体。在 NT2-N 细胞中观察到神经元特异性的电压敏感 Na+ 电流，但在 NT2 细胞中未观察到，这表明 NT2-N 细胞实际上具有神经元的功能。谷氨酸受体激动剂、N-甲基-D-天冬氨酸 (NMDA) 和红藻氨酸会诱发全细胞电流。此外，γ-氨基丁酸 (GABA) 诱发电流，而选择性 GABAA 受体拮抗剂荷包牡丹碱可抑制电流。在由外向外的贴片中，GABA 引发具有两类斜率电导（26 和 50 pS）的单通道电流。然而，ACh、血清素或多巴胺 NT2-N 细胞不会诱导电流，因此表达中枢神经系统中至少两种类型的主要兴奋性和抑制性神经递质受体：谷氨酸和 GAGAA 受体，表明这些受体在大脑发育早期阶段的神经传递中发挥着至关重要的作用。
NT2 cells, a human teratocarcinoma cell line, are shown to be differentiated in neuron-like cells (NT2-N cells) by treatment with retinoic acid. The present study identified the neurotransmitter receptors expressed in NT2-N cells using patch-clamp recording. Voltage-sensitive Na+ currents, which are specific for neurons, were observed in NT2-N cells but not in NT2 cells, suggesting that NT2-N cells actually function as neurons. Glutamate receptor agonists, N-methyl-D-aspartate (NMDA) and kainate, evoked whole-cell currents. In addition, gamma-aminobutyric acid (GABA) evoked currents and the currents were inhibited by the selective GABAA receptor antagonist, bicuculline. In outside-out patches, GABA elicited single channel currents with two classes of the slope conductance (26 and 50 pS). No current, however, was induced by ACh, serotonin, or dopamine NT2-N cells, thus, express at least two types of the major excitatory and inhibitory neurotransmitter receptor in the central nervous system, the glutamate and GAGAA receptors, suggesting that these receptors have a crucial role in neurotransmission from the earlier stage of the brain development.
DOI：
10.1073/pnas.90.6.2174
发表时间：
1993-03
影响因子：
11.1
作者：
D. P. Younkin;Cha Min Tang;M. Hardy;Usha Rani Reddy;Qing Yao Shi;Samuel J. Pleasure;Virginia M.-Y. Lee;David E Pleasure
通讯作者：
D. P. Younkin;Cha Min Tang;M. Hardy;Usha Rani Reddy;Qing Yao Shi;Samuel J. Pleasure;Virginia M.-Y. Lee;David E Pleasure
γ-氨基丁酸A受体的结构和功能。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
DeLorey,TM;Olsen,RW
通讯作者：
Olsen,RW
视黄酸受体γ的过度表达直接诱导人胚胎癌细胞的终末分化。
DOI：
--
发表时间：
1995
期刊：
Oncogene.
影响因子：
--
作者：
Moasser,MM;Reuter,VE;Dmitrovsky,E
通讯作者：
Dmitrovsky,E