Localization and Distribution of Platelet Activating Factor Receptors in the Mouse Ovary and Oviduct During the Estrous Cycle and Early Pregnancy
Localization and Distribution of Platelet Activating Factor Receptors in the Mouse Ovary and Oviduct During the Estrous Cycle and Early Pregnancy
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动情周期和妊娠早期小鼠卵巢和输卵管中血小板激活因子受体的定位和分布
DOI：
10.1111/j.8755-8920.2001.450210.x
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发表时间：
2001
影响因子：
3.6
通讯作者：
M. Legge
中科院分区：
文献类型：
--
作者：
G. Lash;M. Legge
问题：本研究测定血小板活化因子（PAF）与卵巢和输卵管组织的结合，以确定PAF-组织相互作用的位置。
方法：采用冷冻切片和放射自显影法，对发情周期和受孕后第1、4和7天（pc）采集的小鼠卵巢和输卵管进行[ H]PAF结合分析
。动情期和卵巢后壁的PAF结合率无显著性差异（P>0.05），而动情前期和动情期的PAF结合率有显著性差异（P<0.001）。卵巢基质PAF结合率在动情期显著高于动情后期（P<0.001），动情前期无PAF结合。PAF与动情期卵泡的结合率显著高于动情前期和动情后期（P<0.001）。动情期输卵管基质、内上皮和管腔PAF结合率均显著升高（P<0.001）。在交配后第1天和第7天，所有卵巢组织都有最大的PAF结合，而交配后第4天除黄体结合率显著降低外，其余卵巢组织均未结合PAF（P<0.001）。PAF的输卵管结合在pc第1天最大
。卵巢和输卵管PAF受体的表达与胚胎PAF合成高峰相对应，为PAF-PAF受体介导的早孕信号传导系统奠定了基础。
PROBLEM: This study determined platelet activating factor (PAF) binding to ovaries and oviduct tissues to identify the location of PAF–tissue interaction.
METHOD: Mouse ovaries and oviducts taken during the estrous cycle and days 1, 4 and 7 post‐conception (pc) were analyzed by [ H]PAF binding using frozen sections and autoradiography.
RESULTS: For the outer epithelium of estrous and metestrous ovaries there was no significant difference in PAF binding (P>0.05); however, both stages were significantly different from proestrous (P<0.001). Ovarian stroma PAF binding was significantly higher (P<0.001) at estrous than metestrous, with no PAF binding at proestrous. Binding of PAF to estrous ovarian follicles was significantly greater (P<0.001) than at proestrous and metestrous. Estrous oviduct binding of PAF was significantly increased for stroma, inner epithelium and lumen (P<0.001 in all cases). At days 1 and 7 pc, all ovarian tissues had the greatest PAF binding with day 4 pc failing to bind PAF except for a significant decrease in corpora luteal binding (P<0.001). Oviduct binding of PAF was greatest at day 1 pc.
CONCLUSION: Ovarian and oviduct PAF receptor expression corresponds to peak embryo PAF synthesis and establishes the basis for PAF–PAF receptor mediated early pregnancy signaling system.
促性腺激素引发的未成熟大鼠排卵期间卵巢血小板活化因子减少。
影响因子：
3.6
作者：
Espey,LL;Tanaka,N;Woodard,DS;Harper,MJ;Okamura,H
通讯作者：
Okamura,H
兔妊娠早期血小板活化因子与输卵管膜的结合。
DOI：
--
发表时间：
1992
期刊：
Journal of lipid mediators
影响因子：
--
作者：
Yang,YQ;Kudolo,GB;Harper,MJ
通讯作者：
Harper,MJ