Coherence analysis of the calcium activity of putative astrocytic and neuronal cells on the L5 ventral horn and neural output in activated lumbar CPG networks
Coherence analysis of the calcium activity of putative astrocytic and neuronal cells on the L5 ventral horn and neural output in activated lumbar CPG networks
复制标题
L5 腹角上假定的星形胶质细胞和神经元细胞的钙活性以及激活的腰椎 CPG 网络中的神经输出的一致性分析
DOI：
10.1016/j.neulet.2021.136421
复制
发表时间：
2022
影响因子：
2.5
通讯作者：
Okada Yasumasa
中科院分区：
文献类型：
--
作者：
Yazawa Itaru;Okazaki Shuntaro;Yokota Shigefumi;Takeda Kotaro;Fukushi Isato;Yoshizawa Masashi;Onimaru Hiroshi;Okada Yasumasa
星形胶质细胞被认为在提供脊髓结构和维持有效的突触功能和代谢方面起着至关重要的作用，因为它们的精细过程包裹着神经元的突触，并在中枢神经系统（CNS）内形成许多神经元网络。为了研究分布在腹角的假定星形胶质细胞和假定神经元是否在腰椎运动中枢模式发生器（CPG）网络的调节中发挥作用，我们使用细胞外记录和光学成像技术，在激活0-2日龄大鼠离体L1-L5脊髓准备物的同时，记录了左L5腹根的神经输出和L5腹角假定星形胶质细胞和神经元的钙活性。光学测量检测到的细胞在所有实验条件下，即(1)5-HT + NMDA, (2) TTX, (3) TTX + Low K+，都表现出荧光强度的变化。这些细胞使用内部基于matlab的程序半自动识别，根据细胞分类，即荧光强度变化的增加或减少（ΔF/F0），以及基于其体细胞大小的主观判断，推定为星形胶质细胞和神经元。根据假定的星形胶质细胞和假定的神经元的钙活性计算相干性及其相位，并使用内部基于matlab的程序计算有效运动时的神经输出。我们发现，通过低K+激活的星形胶质细胞的数量往往与使用蛋白酶激活受体1 （PAR1）选择性激动剂TFLLR- nh2 （TFLLR）激活的星形胶质细胞的数量没有区别。此外，一些假定的星形胶质细胞和神经元的钙活性在低于0.5 Hz的频率范围内与运动样活动同步，细胞钙活性峰值与运动样活动之间的时间滞后范围为- 1000至+ 1000 ms。这些发现可能表明，左L5腹角的这些假定的星形胶质细胞和神经元需要- 1000至+ 1000 ms才能与腰椎CPG网络通信，并在激活的腰椎CPG网络中维持有效的突触功能和代谢。这一发现表明，L5腹角的星形细胞和神经元细胞可能通过激活第一至第五腰椎的CPG网络，参与产生运动样活动的节律和模式。
Astrocytes are thought to play a crucial role in providing structure to the spinal cord and maintaining efficient synaptic function and metabolism because their fine processes envelop the synapses of neurons and form many neuronal networks within the central nervous system (CNS). To investigate whether putative astrocytes and putative neurons distributed on the ventral horn play a role in the modulation of lumbar locomotor central pattern generator (CPG) networks, we used extracellular recording and optical imaging techniques and recorded the neural output from the left L5 ventral root and the calcium activity of putative astrocytes and neurons in the L5 ventral horn at the same time when activating an isolated L1–L5 spinal cord preparation from rats aged 0–2 days. Optical measurements detected cells that showed a fluorescence intensity change under all experimental conditions, namely, (1) 5-HT + NMDA, (2) TTX, and (3) TTX + Low K+. These cells were semiautomatically identified using an in-house MATLAB-based program, as putative astrocytes and neurons according to the cell classification, i.e., increased or decreased fluorescence intensity change (ΔF/F0), and subjective judgment based on their soma size. Coherence and its phase were calculated according to the calcium activity of the putative astrocytes and putative neurons, and neural output was calculated during fictive locomotion with in-house MATLAB-based programs. We found that the number of putative astrocytes activated by applying low K+tends not to differ from that activated by applying the protease-activated receptor 1 (PAR1) selective agonist TFLLR-NH2(TFLLR). Moreover, the calcium activity of several putative astrocytes and neurons synchronized with locomotor-like activity at a frequency range below 0.5 Hz and the time lag between peaks of cellular calcium activity and locomotor-like activity ranged from −1000 to + 1000 ms. These findings presumably indicates that these putative astrocytes and neurons in the left L5 ventral horn require −1000 to + 1000 ms to communicate with lumbar CPG networks and maintain efficient synaptic function and metabolism in activated lumbar CPG networks. This finding suggests the possibility that putative astrocytic and neuronal cells in the L5 ventral horn contribute to generating the rhythms and patterns of locomotor-like activity by activated CPG networks in the first to fifth lumbar spinal cord.
登录
查看更多内容
影响因子：
4
作者：
Haertel, Kai;Singaravelu, Karthika;Deitmer, Joachim W.
通讯作者：
Deitmer, Joachim W.
影响因子：
3.7
作者：
Acton D;Miles GB
通讯作者：
Miles GB
腺苷介导的新生小鼠腹角中间神经元和脊髓运动神经元的调节
影响因子：
2.5
作者：
Emily C. Witts;Filipe Nascimento;G. Miles
通讯作者：
G. Miles
影响因子：
25
作者：
McLean, David L.;Masino, Mark A.;Koh, Ingrid Y. Y.;Lindquist, W. Brent;Fetcho, Joseph R.
通讯作者：
Fetcho, Joseph R.
对猫不受限制运动时后肢肌肉活动的肌电图分析。
DOI：
--
发表时间：
1969
期刊：
Acta Physiologica Scandinavica
影响因子：
--
作者：
I. Engberg;A. Lundberg
通讯作者：
A. Lundberg