Physical and linkage mapping of human chromosome 17 loci to dog chromosomes 9 and 5.
Physical and linkage mapping of human chromosome 17 loci to dog chromosomes 9 and 5.
复制标题
人类 17 号染色体基因座与狗 9 号和 5 号染色体的物理和连锁图谱。
DOI：
10.1006/geno.1997.4723
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Patterson,DF
中科院分区：
文献类型：
--
作者：
Werner,P;Raducha,MG;Prociuk,U;Henthorn,PS;Patterson,DF
狗的基因组图谱还处于早期阶段。在这里，我们说明了一种利用人类和小鼠的同源性信息、品种间杂交/回交以及分离包含基因特异序列和简单序列重复多态的狗基因组克隆的方法，对狗的1型锚定(基因)座位进行联合物理和连锁定位。将人类染色体17q(HSA17q)中的11个基因座定位于狗9号染色体着丝粒的三分之二(CFA9)，这是一条中等大小的顶着丝粒染色体：P4HB、GALK1、TK1、GH1、MYL4、BRCA1、RARA、THRA1、MPO、NF1和CRYBA1。其中8个也被定位在跨度38.6 cM的连锁图谱上。基于荧光原位杂交和连锁定位，CFA9上的基因序列与HSA17q和小鼠11号染色体(MMU11)上的同源基因序列相似，而狗的基因序列与着丝粒相反。犬基因座GALK1、TK1、GH1、MYL4、THRA1和RARA在CFA9着丝粒末端附近构成一个紧密连锁的群体，遗传距离仅为4.7 cM。犬NF1和CRYBA1位于远端，靠近Giemsa带的下缘，毗邻CFA9的远端三分之一。NF1和CRYBA1松散地连接到更着丝粒的基团(31.2 cM)。在CFA9三分之一的端粒上没有发现HSA17基因。用人类完整的17号染色体探针绘制狗的染色体显示，只与CFA9近端三分之二的片段杂交，这与远端三分之一对应于一个或多个其他人类染色体片段的结论一致。位于HSA17p上的两个基因座GLUT4和PMP22被FISH定位到狗的5号染色体上，该区域也被整个人类17号染色体所识别，表明着丝粒上HSA17的共线同源性被破坏。
Genome mapping in the dog is in its early stages. Here we illustrate an approach to combined physical and linkage mapping of type 1 anchor (gene) loci in the dog using information on syntenic homology from human and mouse, an interbreed cross/backcross, and a strategy for isolation of dog genomic clones containing both gene-specific sequences and simple sequence repeat polymorphisms. Eleven gene loci from human chromosome 17q (HSA17q) were mapped to the centromeric two-thirds of dog chromosome 9 (CFA9), an acrocentric chromosome of medium size: P4HB, GALK1, TK1, GH1, MYL4, BRCA1, RARA, THRA1, MPO, NF1, and CRYBA1. Eight of these were also positioned on a linkage map spanning 38.6 cM. Based on combined fluorescencein situhybridization and linkage mapping, the gene order on CFA9 is similar to that of the homologous genes on HSA17q and mouse chromosome 11 (MMU11), but in the dog the gene order is inverted with respect to the centromere. Canine loci, GALK1, TK1, GH1, MYL4, THRA1, and RARA constitute a closely linked group near the centromeric end of CFA9, spanning a genetic distance of only 4.7 cM. Canine NF1 and CRYBA1 lie distally, near the lower border of the Giemsa band adjacent to the distal one-third of CFA9. NF1 and CRYBA1 are loosely linked to the more centromeric group (31.2 cM). No HSA17 genes were found on the telomeric one-third of CFA9. Painting of dog chromosomes with a human whole chromosome 17 probe showed hybridization with only the proximal two-thirds of CFA9, consistent with the conclusion that the distal one-third corresponds to a segment or segments of other human chromosomes. Two loci, GLUT4 and PMP22, located on HSA17p, were mapped by FISH to dog chromosome 5 in a region also identified by the whole human chromosome 17 paint, indicating disruption of HSA17 syntenic homology at the centromere.
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DOI：
10.1159/000132079
发表时间：
1984
期刊：
Cytogenetics and cell genetics
影响因子：
--
作者：
L. Cannizzaro;B. Emanuel
通讯作者：
L. Cannizzaro;B. Emanuel
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs
犬和人近端 Xq 的比较图谱以及犬 X 连锁严重联合免疫缺陷的遗传分析。
影响因子：
4.4
作者：
Deschenes,SM;Puck,JM;Dutra,AS;Somberg,RL;Felsburg,PJ;Henthorn,PS
通讯作者：
Henthorn,PS
影响因子：
56.9
作者：
EVANS, RM
通讯作者：
EVANS, RM
使用简并引物对来自粘粒插入物的 (CA)n 微卫星进行循环测序。
影响因子：
2.7
作者：
G. Hawkins;M. Bishop;S. Kappes;C. Beattie
通讯作者：
C. Beattie